Dokument: Development and Standardization of the CellDrumTM: A Biofunctionalized Ultra-Thin PDMS Membrane Sensor for Advanced Cell Culture and Mechanobiological Testing

Titel:	Development and Standardization of the CellDrumTM: A Biofunctionalized Ultra-Thin PDMS Membrane Sensor for Advanced Cell Culture and Mechanobiological Testing
URL für Lesezeichen:	https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=74223
URN (NBN):	urn:nbn:de:hbz:061-20261013-165504-5
Kollektion:	Dissertationen
Sprache:	Englisch
Dokumententyp:	Wissenschaftliche Abschlussarbeiten » Dissertation
Medientyp:	Text
Autor:	Uysal, Karya [Autor]
Dateien:	[Dateien anzeigen] Adobe PDF [Details] 4,18 MB in einer Datei [ZIP-Datei erzeugen] Dateien vom 10.09.2026 / geändert 10.09.2026
Beitragende:	Prof. Dr. Teusch, Nicole [Gutachter] Prof. Dr. Dr. Temiz-Artmann, Aysegül [Gutachter]
Dewey Dezimal-Klassifikation:	600 Technik, Medizin, angewandte Wissenschaften » 610 Medizin und Gesundheit
Beschreibung:	This work focuses on the optimization, characterization, and validation of the CellDrumTM technology. A CellDrum is a physical cell force sensor consisting of an ultra-thin, flexible PDMS membrane that seals one side of a cylindrical base body (applicator) in a water-tight manner. When filled with cell culture medium, the membrane bends slightly downward, and this deflection can be measured. Cell and tissue layers cultivated on the membrane form a composite membrane consisting of living cells, extracellular matrix and PDMS. The tensile forces in the connected cell layer change the mechanical properties of this composite membrane and thus its deflection. The ratio of the number of cells causing the tensile stress to the PDMS membrane thickness and its preload determines the sensitivity of these cell force sensors. The CellDrum technology was developed for advanced mechanobiological testing. The study was divided into three experimental phases. Phase 1 optimized the CellDrumTM manufacturing process to determine critical parameters such as membrane thickness, uniformity, and mechanical stability, with quality control measures including visual inspection, interferometric thickness measurement and membrane deflection detection performed to increase reproducibility and consistency. Phase 2 validated the biological functionality of CellDrumTM membranes through "wound healing" assays using skin fibroblasts derived from young and aged human donors, where "wound healing" was defined as the capacity of cultured cell monolayers to migrate and proliferate across an artificially generated cell-free gap with progressive reduction in gap area serving as a quantitative measure of cellular repair processes monitored by fluorescence microscopy, revealing age-dependent differences in "wound healing" behaviour with mechanical stimulation improving "wound" closing in "younger" cells but showing less favourable or adverse effects in "older" cell samples. Phase 3 measured contractile responses of differentiated primary human aortic smooth muscle cell (HAOSMC) and primary human aortic endothelial cell (HAOEC) co-cultures after exposure to noradrenaline and ionomycin using the Cell Force Analyzer (CFArefer), where HAOSMC monolayers exhibited clear, measurable contractility while co-culture data showed more complex, nonlinear patterns, and ionomycin confirmed the functional contractility of the model. Overall, this work demonstrates that the CellDrumTM platform may serve as a reproducible, flexible, and physiologically relevant tool for mechanobiology research, with potential applications in tissue engineering, pharmacological testing, and regenerative medicine.
Lizenz:	Dieses Werk ist lizenziert unter einer Creative Commons Namensnennung 4.0 International Lizenz
Fachbereich / Einrichtung:	Mathematisch- Naturwissenschaftliche Fakultät » WE Pharmazie » Pharmazeutische Biologie und Biotechnologie
Dokument erstellt am:	13.10.2026
Dateien geändert am:	13.10.2026
Promotionsantrag am:	22.11.2022
Datum der Promotion:	22.06.2026