Dokument: Impact of the nisin modification machinery on the transport kinetics of NisT
| Titel: | Impact of the nisin modification machinery on the transport kinetics of NisT | |||||||
| URL für Lesezeichen: | https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=71519 | |||||||
| URN (NBN): | urn:nbn:de:hbz:061-20251124-115210-5 | |||||||
| Kollektion: | Publikationen | |||||||
| Sprache: | Englisch | |||||||
| Dokumententyp: | Wissenschaftliche Texte » Artikel, Aufsatz | |||||||
| Medientyp: | Text | |||||||
| Autoren: | Lagedroste, Marcel [Autor] Reiners, Jens [Autor] Smits, Sander H. J. [Autor] Schmitt, Lutz [Autor] | |||||||
| Dateien: |
| |||||||
| Beschreibung: | Lanthipeptides are ribosomally synthesized and post-translationally modified peptides containing dehydrated amino acids and (methyl-)lanthionine rings. One of the best-studied examples is nisin produced by Lactococcus lactis. Nisin is synthesized as a precursor peptide comprising of an N-terminal leader peptide and a C-terminal core peptide. Amongst others, the leader peptide is crucial for enzyme recognition and acts as a secretion signal for the ABC transporter NisT that secretes nisin in a proposed channeling mechanism. Here, we present an in vivo secretion analysis of this process in the presence and absence of the nisin maturation machinery, consisting of the dehydratase NisB and the cyclase NisC. Our determined apparent secretion rates of NisT show how NisB and NisC modulate the transport kinetics of NisA. Additional in vitro studies of the detergent-solubilized NisT revealed how these enzymes and the substrates again influence the activity of transporter. In summary, this study highlights the pivotal role of NisB for NisT in the secretion process. | |||||||
| Rechtliche Vermerke: | Originalveröffentlichung:
Lagedroste, M., Reiners, J., Smits, S. H. J., & Schmitt, L. (2020). Impact of the nisin modification machinery on the transport kinetics of NisT [OnlineRessource]. Scientific Reports, 10, Article 12295. https://doi.org/10.1038/s41598-020-69225-2 | |||||||
| Lizenz: |  Dieses Werk ist lizenziert unter einer Creative Commons Namensnennung 4.0 International Lizenz | |||||||
| Fachbereich / Einrichtung: | Mathematisch- Naturwissenschaftliche Fakultät | |||||||
| Dokument erstellt am: | 24.11.2025 | |||||||
| Dateien geändert am: | 24.11.2025 |
