Dokument: Functional omics analyses reveal only minor effects of microRNAs on human somatic stem cell differentiation
| Titel: | Functional omics analyses reveal only minor effects of microRNAs on human somatic stem cell differentiation | |||||||
| URL für Lesezeichen: | https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=71341 | |||||||
| URN (NBN): | urn:nbn:de:hbz:061-20251113-112744-6 | |||||||
| Kollektion: | Publikationen | |||||||
| Sprache: | Englisch | |||||||
| Dokumententyp: | Wissenschaftliche Texte » Artikel, Aufsatz | |||||||
| Medientyp: | Text | |||||||
| Autoren: | Schira-Heinen, Jessica [Autor] Czapla, Agathe [Autor] Hendricks, Marion [Autor] Kloetgen, Andreas [Autor] Wruck, Wasco [Autor] Adjaye, James [Autor] Kögler, Gesine [Autor] Müller, Hans Werner [Autor] Stühler, Kai [Autor] Trompeter, Hans-Ingo [Autor] | |||||||
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| Beschreibung: | The contribution of microRNA-mediated posttranscriptional regulation on the final proteome in differentiating cells remains elusive. Here, we evaluated the impact of microRNAs (miRNAs) on the proteome of human umbilical cord blood-derived unrestricted somatic stem cells (USSC) during retinoic acid (RA) differentiation by a systemic approach using next generation sequencing analysing mRNA and miRNA expression and quantitative mass spectrometry-based proteome analyses. Interestingly, regulation of mRNAs and their dedicated proteins highly correlated during RA-incubation. Additionally, RA-induced USSC demonstrated a clear separation from native USSC thereby shifting from a proliferating to a metabolic phenotype. Bioinformatic integration of up- and downregulated miRNAs and proteins initially implied a strong impact of the miRNome on the XXL-USSC proteome. However, quantitative proteome analysis of the miRNA contribution on the final proteome after ectopic overexpression of downregulated miR-27a-5p and miR-221-5p or inhibition of upregulated miR-34a-5p, respectively, followed by RA-induction revealed only minor proportions of differentially abundant proteins. In addition, only small overlaps of these regulated proteins with inversely abundant proteins in non-transfected RA-treated USSC were observed. Hence, mRNA transcription rather than miRNA-mediated regulation is the driving force for protein regulation upon RA-incubation, strongly suggesting that miRNAs are fine-tuning regulators rather than active primary switches during RA-induction of USSC. | |||||||
| Rechtliche Vermerke: | Originalveröffentlichung:
Schira-Heinen, J., Czapla, A., Hendricks, M., Klötgen, A., Wruck, W., Adjaye, J., Kögler, G., Müller, H. W., Stühler, K., & Trompeter, H.-I. (2020). Functional omics analyses reveal only minor effects of microRNAs on human somatic stem cell differentiation [OnlineRessource]. Scientific Reports, 10, Article 3284. https://doi.org/10.1038/s41598-020-60065-8 | |||||||
| Lizenz: |  Dieses Werk ist lizenziert unter einer Creative Commons Namensnennung 4.0 International Lizenz | |||||||
| Fachbereich / Einrichtung: | Mathematisch- Naturwissenschaftliche Fakultät Medizinische Fakultät | |||||||
| Dokument erstellt am: | 13.11.2025 | |||||||
| Dateien geändert am: | 13.11.2025 |
