Dokument: Method Development in Nanobiophysics
| Titel: | Method Development in Nanobiophysics | |||||||
| URL für Lesezeichen: | https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=66402 | |||||||
| URN (NBN): | urn:nbn:de:hbz:061-20240722-112121-4 | |||||||
| Kollektion: | Dissertationen | |||||||
| Sprache: | Englisch | |||||||
| Dokumententyp: | Wissenschaftliche Abschlussarbeiten » Dissertation | |||||||
| Medientyp: | Text | |||||||
| Autor: | Kuckla, Daniel Alexander [Autor] | |||||||
| Dateien: |
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| Beitragende: | Prof. Dr. Monzel, Cornelia [Gutachter] Prof. Dr. Getzlaff, Mathias [Gutachter] | |||||||
| Dokumententyp (erweitert): | Dissertation | |||||||
| Dewey Dezimal-Klassifikation: | 500 Naturwissenschaften und Mathematik » 530 Physik | |||||||
| Beschreibung: | During this PhD, two projects were pursued.
Development of a Setup for Magnetic Hyperthermia Magnetic hyperthermia describes heat dissipation by nanoparticles subjected to alternating magnetic fields. Due to the nanoscale localized heating, this phenomenon has a high potential for biological and medical applications. These include but are not limited to localized heating of cancerous tissue, causing conformational changes of heat sensitive molecules, and targeted drug release. However, the generation of temporally stable alternating magnetic fields remains a challenge. In this project, a robust, microscope-mountable setup for generating an alternating magnetic field is developed. For this purpose, different electrical components are tested to maximize the efficiency. The resulting setup is capable of maintaining a 100 kHz alternating magnetic field with an amplitude of 50 mT over a period of 30 min, and offers to apply fast heating-cooling cycles. An elaborate cooling system is introduced to minimize the effect of parasitic sample heating. To test the system, Synomag R© 70 particles, Bionized Nano Ferrite particles, and magnetosomes from magnetotactic bacteria (Magnetospirillum gryphiswaldense) are characterized in their physical and magnetic properties. The particles are compared regarding their core structure, hydrodynamic radius, surface charge, and alternating-current-susceptibility. The particles are then used to demonstrate the heating capability of the hyperthermia setup. Track Separation in Single Particle Tracking Intracellular transport is vital for cell division and metabolism. Single particle tracking is an essential tool for understanding these cellular processes. Today single-particle tracking can be performed on multiple particles simultaneously. Due to the high amount of data generated, computer algorithms are used to evaluate the results. In this project, an algorithm is developed which enables the separation of tracks in segments with different modes of transportation. This is accomplished via the point density of the recorded track, as criterion. To test the developed algorithm, it is applied to tracking data of endosomes captured on an in-house built tracking setup and evaluated using the tracking algorithm u-Track. The resulting separation is then compared to the established analysis via mean square displacement theory, verifying the viability of the developed approach. | |||||||
| Lizenz: |  Dieses Werk ist lizenziert unter einer Creative Commons Namensnennung 4.0 International Lizenz | |||||||
| Fachbereich / Einrichtung: | Mathematisch- Naturwissenschaftliche Fakultät » WE Physik » Experimentalphysik | |||||||
| Dokument erstellt am: | 22.07.2024 | |||||||
| Dateien geändert am: | 22.07.2024 | |||||||
| Promotionsantrag am: | 24.08.2023 | |||||||
| Datum der Promotion: | 20.11.2023 |
