Dokument: Uptake and subcellular distribution of carbon nanoparticles
| Titel: | Uptake and subcellular distribution of carbon nanoparticles | |||||||
| URL für Lesezeichen: | https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=62899 | |||||||
| URN (NBN): | urn:nbn:de:hbz:061-20230619-110945-7 | |||||||
| Kollektion: | Dissertationen | |||||||
| Sprache: | Englisch | |||||||
| Dokumententyp: | Wissenschaftliche Abschlussarbeiten » Dissertation | |||||||
| Medientyp: | Text | |||||||
| Autor: | Wimmenauer, Christian [Autor] | |||||||
| Dateien: |
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| Beitragende: | Prof. Dr. Thomas Heinzel [Betreuer/Doktorvater] [im Online-Personal- und -Vorlesungsverzeichnis LSF anzeigen] Prof. Dr. Cornelia Monzel [Gutachter] | |||||||
| Dewey Dezimal-Klassifikation: | 500 Naturwissenschaften und Mathematik » 530 Physik | |||||||
| Beschreibung: | This thesis treats the characterization of carbon nanoparticles regarding their subcellular distribution and cell-specific uptake rate to enable their use in biomedical applications or basic research in the area of life science. Four manuscripts resulting from this work report our main findings.
As discussed in Paper I, we found that carbon nanoparticles derived from citric acid and diethylenetriamine in a microwave-assisted bottom-up synthesis accumulate mainly in the lysosomes of the breast cancer cell line MCF-7 after 48 h of exposure. A workflow for object-based colocalization analysis of moving point-like objects was developed and applied to microscopy data acquired via Airy scanning microscopy in super-resolution mode. This workflow, which is based on point pattern analysis, was successfully validated with a positive control and a negative control and allows the assessment of the statistical significance of the results by the comparison with the results from Monte-Carlo simulations under a model assumption. This method overcomes common shortcomings of many traditional colocalization techniques since the analyzed objects do not need to overlap in both channels, and inference on a solid statistical basis is possible. Paper II investigated the differential uptake of blood cells derived from healthy donors compared to samples from patients with acute myeloid leukemia (AML). A significantly smaller uptake of the same carbon nanoparticles was reported for the CD33+ and CD34+ subsets of samples from AML patients compared to the CD33+ and CD34+ subsets of healthy donors. In good agreement with previous findings with other cells, the nanoparticles showed perinuclear accumulation in the AML cell line HL-60 on confocal microscopy images that may correspond to an immobile pool of lysosomes near the microtubule organizing center. In Paper III, we attempted to modify the cell-specific uptake of the employed carbon nanoparticles via modification with sugar monomers and sugar-decorated oligomers. While we did not observe a cell-type specific uptake, we found that the monomerfunctionalized nanoparticles showed a two- to threefold increased uptake rate across all cell types compared to the non-functionalized and oligomer-coupled nanoparticles. Regarding their subcellular localization, no difference between the nanoparticle species was observed since all conditions showed significant accumulation in the lysosomes, as revealed by colocalization experiments. Finally, Paper IV reports the results from tight-binding simulations of multilayer graphene quantum dots. The calculations reveal a redshift with an increasing number of layers, as apparent in the optical absorption spectrum and the decrease of the energy gap. Furthermore, the edge type and the geometry of the multilayer graphene quantum dot play a dominant role not only in the lateral size dependence of the energy spectrum but also in the dependence on the particle’s number of layers. | |||||||
| Lizenz: |  Dieses Werk ist lizenziert unter einer Creative Commons Namensnennung 4.0 International Lizenz | |||||||
| Fachbereich / Einrichtung: | Mathematisch- Naturwissenschaftliche Fakultät » WE Physik » Physik der kondensierten Materie | |||||||
| Dokument erstellt am: | 19.06.2023 | |||||||
| Dateien geändert am: | 19.06.2023 | |||||||
| Promotionsantrag am: | 21.03.2023 | |||||||
| Datum der Promotion: | 05.06.2023 |
