Dokument: siRNA Delivery via Cross‐Linked Gelatin Microparticles Enables Targeted Modulation of Osteogenic‐Vascular Cross‐Talk: An Advanced Human 3D in Vitro Test System for Therapeutic siRNA
| Titel: | siRNA Delivery via Cross‐Linked Gelatin Microparticles Enables Targeted Modulation of Osteogenic‐Vascular Cross‐Talk: An Advanced Human 3D in Vitro Test System for Therapeutic siRNA | |||||||
| URL für Lesezeichen: | https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=73543 | |||||||
| URN (NBN): | urn:nbn:de:hbz:061-20260610-102716-4 | |||||||
| Kollektion: | Publikationen | |||||||
| Sprache: | Englisch | |||||||
| Dokumententyp: | Wissenschaftliche Texte » Artikel, Aufsatz | |||||||
| Medientyp: | Text | |||||||
| Autoren: | Demir, Burak [Autor] Hacker, Michael C. [Autor] Mitrach, Franziska [Autor] Kubat, Jonas [Autor] Simm, Stefan [Autor] Springwald, Alexandra H. [Autor] Liebezeit, Anton [Autor] Schulz‐Siegmund, Michaela [Autor] | |||||||
| Dateien: |
| |||||||
| Stichwörter: | bone regeneration , siRNA release , tissue engineering , spheroids , microspheres | |||||||
| Beschreibung: | Small interfering RNAs (siRNAs) have drawn particular attention for their ability to transiently and sequence-specifically silence target genes, not only for systemic but also for localized application. For bone regeneration, targeting inhibitory regulators by siRNAs offers opportunities to improve osteogenic–angiogenic coupling. Conventional experimental models often oversimplify this interaction as they fail to capture these multicellular tissue dynamics. To address this, we established a human three-dimensional co-culture model composed of osteogenic and vascular microtissues embedded in fibrin hydrogels to investigate siRNA effects on microtissue interaction. Local siRNA delivery to microtissues was achieved by oligomer-stabilized calcium phosphate nanoparticles (CaP-NP) loaded onto cross-linked gelatin microparticles (cGM). siRNA/CaP-NP-loaded cGM were assembled with human mesenchymal stem cells (hMSCs) to microtissues. This approach was demonstrated by silencing two antagonists with distinct expression profiles: Chordin, a low-abundance BMP inhibitor, and WWP-1, a highly expressed E3 ligase. Only Chordin siRNA improved the osteogenic-vascular
cross-talk, whereas WWP-1 siRNA effects were limited to osteogenic effects. Next-generation sequencing (NGS) supported these results. | |||||||
| Rechtliche Vermerke: | Originalveröffentlichung:
Mitrach, F., Kubat, J., Simm, S., Springwald, A. H., Demir, B., Liebezeit, A., Hacker, M., & Schulz‐Siegmund, M. (2026). siRNA Delivery via Cross‐Linked Gelatin Microparticles Enables Targeted Modulation of Osteogenic‐Vascular Cross‐Talk: An Advanced Human 3D in Vitro Test System for Therapeutic siRNA. Advanced Healthcare Materials, 15(13), Article e04773. https://doi.org/10.1002/adhm.202504773 | |||||||
| Lizenz: |  Dieses Werk ist lizenziert unter einer Creative Commons Namensnennung 4.0 International Lizenz | |||||||
| Fachbereich / Einrichtung: | Mathematisch- Naturwissenschaftliche Fakultät | |||||||
| Dokument erstellt am: | 10.06.2026 | |||||||
| Dateien geändert am: | 10.06.2026 |
