Dokument: The Role of Metabolite Transport across Organellar Membranes in Plants and Algae
| Titel: | The Role of Metabolite Transport across Organellar Membranes in Plants and Algae | |||||||
| URL für Lesezeichen: | https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=72460 | |||||||
| URN (NBN): | urn:nbn:de:hbz:061-20260309-083246-2 | |||||||
| Kollektion: | Dissertationen | |||||||
| Sprache: | Englisch | |||||||
| Dokumententyp: | Wissenschaftliche Abschlussarbeiten » Dissertation | |||||||
| Medientyp: | Text | |||||||
| Autor: | Plett, Anastasija [Autor] | |||||||
| Dateien: |
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| Beitragende: | Prof. Dr. Weber, Andreas P.M. [Gutachter] Prof. Dr. Kopriva, Stanislav [Gutachter] | |||||||
| Dewey Dezimal-Klassifikation: | 500 Naturwissenschaften und Mathematik » 580 Pflanzen (Botanik) | |||||||
| Beschreibung: | Compartmentalization is a key feature of eukaryotic cells, creating distinct areas for various metabolic processes and enabling the formation of chemical gradients and optimal environments for enzymes. To enable exchange of metabolites and connect biochemical pathways across several organellar membranes, transporters, channels, and pores are essential.
Peroxisomes are comparatively small but essential organelles in all eukaryotic organisms. They are involved in several processes such as beta-oxidation, photorespiration and biosynthesis of secondary and defense molecules. Since many of those pathways are shared between organelles, peroxisomes are closely interconnected to those organelles and the cellular metabolism. The peroxisomal membrane contains several active and passive transporters as well as channels (reviewed in Manuscript I). One of the most abundant peroxisomal membrane proteins is PEROXISOMAL MEMBRANE PROTEIN 22 (PMP22) which belongs to the MPV17/PMP22 family. Based on studies of mammalian orthologues, it is proposed to function as a non-selective pore-forming channel. In Manuscript II we analyzed the role of PMP22 in context of beta-oxidation and amino acid metabolism under certain environmental conditions. Transporters are not only important in higher plants but also in microalgae. The green algae Chlamydomonas reinhardtii is a model organism to study photosynthesis. The origin of plastids can be traced back several billion years ago through endosymbiosis. One prime example of plastid evolution from the perspective of transport proteins is the plastidial phosphate translocator (pPT) family. This family is conserved in Archaeplastida and encodes for transporters catalyzing a strict counter exchange carbons in exchange for inorganic phosphate. We analyzed the biochemical and physiological role of two TRIOSE PHOSPHATE TRANSLOCATORS in Chlamydomonas reinhardtii (CreTPT) (Manuscript III). Finally, we provide a detailed protocol for metabolite extraction and analysis for plants exposed to transient environmental conditions (Manuscript IV). | |||||||
| Lizenz: |  Dieses Werk ist lizenziert unter einer Creative Commons Namensnennung 4.0 International Lizenz | |||||||
| Fachbereich / Einrichtung: | Mathematisch- Naturwissenschaftliche Fakultät » WE Biologie » Biochemie der Pflanzen | |||||||
| Dokument erstellt am: | 09.03.2026 | |||||||
| Dateien geändert am: | 09.03.2026 | |||||||
| Promotionsantrag am: | 10.10.2025 | |||||||
| Datum der Promotion: | 26.02.2026 |
