Dokument: CRISPR/Cpf1-mediated editing of PINK1 in induced pluripotent stem cells
| Titel: | CRISPR/Cpf1-mediated editing of PINK1 in induced pluripotent stem cells | |||||||
| URL für Lesezeichen: | https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=72232 | |||||||
| URN (NBN): | urn:nbn:de:hbz:061-20260210-095159-1 | |||||||
| Kollektion: | Publikationen | |||||||
| Sprache: | Englisch | |||||||
| Dokumententyp: | Wissenschaftliche Texte » Artikel, Aufsatz | |||||||
| Medientyp: | Text | |||||||
| Autoren: | Ghodrat, Roohallah [Autor] Hildebrandt, Barbara [Autor] Reichert, Andreas S. [Autor] Ramachandran, Haribaskar [Autor] Binder, Stephanie [Autor] Rossi, Andrea [Autor] | |||||||
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| Beschreibung: | The PTEN induced kinase 1 (PINK1) gene is crucial for mitophagy and mitochondrial quality control. Mutations in the PINK1 gene are associated with several neurological disorders. To decipher the role of PINK1-mediated mitophagy in human induced pluripotent stem cells (hiPSCs) and in their differentiated counterparts, we used CRISPR/Cpf1 and generated a human iPSC line with homozygous out-of-frame deletions by targeting exon 6 of the PINK1 gene. The generated homozygous PINK1 mutant cell line showed normal cell morphology, genomic stability, and expression of classical stem cell markers. Furthermore, the cells can be differentiated efficiently into the three germ layers. | |||||||
| Rechtliche Vermerke: | Originalveröffentlichung:
Ghodrat, R., Ramachandran, H., Hildebrandt, B., Binder, S., Rossi, A., & Reichert, A. (2025). CRISPR/Cpf1-mediated editing of PINK1 in induced pluripotent stem cells. Stem Cell Research, 90, Article 103887. https://doi.org/10.1016/j.scr.2025.103887 | |||||||
| Lizenz: |  Dieses Werk ist lizenziert unter einer Creative Commons Namensnennung 4.0 International Lizenz | |||||||
| Fachbereich / Einrichtung: | Medizinische Fakultät | |||||||
| Dokument erstellt am: | 10.02.2026 | |||||||
| Dateien geändert am: | 10.02.2026 |
