Dokument: Serum-free differentiation platform for the generation of B lymphocytes and natural killer cells from human CD34+ cord blood progenitors
| Titel: | Serum-free differentiation platform for the generation of B lymphocytes and natural killer cells from human CD34+ cord blood progenitors | |||||||
| URL für Lesezeichen: | https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=72012 | |||||||
| URN (NBN): | urn:nbn:de:hbz:061-20260123-125348-3 | |||||||
| Kollektion: | Publikationen | |||||||
| Sprache: | Englisch | |||||||
| Dokumententyp: | Wissenschaftliche Texte » Artikel, Aufsatz | |||||||
| Medientyp: | Text | |||||||
| Autoren: | Bhave, Rigveda [Autor] Kath, Carla-Johanna [Autor] Rüchel, Nadine [Autor] Vasileiou, Eleni [Autor] Jepsen, Vera H. [Autor] Raba, Katharina [Autor] Pandyra, Aleksandra A. [Autor] Pandyra, Aleksandra A. [Autor] Kögler, Gesine [Autor] | |||||||
| Dateien: |
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| Stichwörter: | Culture media , B lymphocytes , Serum-free , Natural , Mesenchymal stem cells , Killer cells , Cord blood , Cell differentiation | |||||||
| Beschreibung: | Pre-clinical research on B and NK cell development relies on murine stromal cell-based systems with reduced physiological relevance and clinical applicability. A serum-free, fully humanized co-culture system utilizing human bone marrow-derived mesenchymal stromal cells (BM-MSCs) was developed to differentiate CB-CD34+ cells towards B and NK cell lineages. Differentiation dynamics were monitored via flow cytometry, with immunophenotypic analysis tracking progression from progenitors to mature cells. The system generated CD19+ IgM+ immature B cells and CD56+ CD16+ NK cells, recapitulating fetal stages of human lymphopoiesis. Serum-free media conditions ensured reproducibility and high overall yield of CD19+ B (35 ± 5.32%) and CD56+ NK (28.46 ± 7.01%) cell progenitors. Flow cytometry identified distinct population peaks, confirming temporal control over differentiation. This clinically relevant platform addresses the limitations of traditional models by providing a more physiologically accurate human microenvironment. The serum-free system supports applications in disease modeling, genotoxic compound screening, and mutational studies of hematopoiesis. By enabling scalable production of B and NK cells it aims to accelerate translational research for immunodeficiencies, cancer immunotherapy, and hematopoietic disorders. | |||||||
| Rechtliche Vermerke: | Originalveröffentlichung:
Bhave, R. N., Kath, C.-J., Rüchel, N., Vasileiou, E., Jepsen, V., Raba, K., Pandyra, A. A., Fischer, U., & Kögler, G. (2025). Serum-free differentiation platform for the generation of B lymphocytes and natural killer cells from human CD34+ cord blood progenitors. Scientific Reports, 15, Article 44132. https://doi.org/10.1038/s41598-025-30732-9 | |||||||
| Lizenz: |  Dieses Werk ist lizenziert unter einer Creative Commons Namensnennung 4.0 International Lizenz | |||||||
| Fachbereich / Einrichtung: | Medizinische Fakultät | |||||||
| Dokument erstellt am: | 23.01.2026 | |||||||
| Dateien geändert am: | 23.01.2026 |
