Dokument: Metabolic Investigations of the Human Liver using Phosphorus-31 Magnetic Resonance Spectroscopy at 3 Tesla
| Titel: | Metabolic Investigations of the Human Liver using Phosphorus-31 Magnetic Resonance Spectroscopy at 3 Tesla | |||||||
| URL für Lesezeichen: | https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=71758 | |||||||
| URN (NBN): | urn:nbn:de:hbz:061-20260129-125445-2 | |||||||
| Kollektion: | Dissertationen | |||||||
| Sprache: | Englisch | |||||||
| Dokumententyp: | Wissenschaftliche Abschlussarbeiten » Dissertation | |||||||
| Medientyp: | Text | |||||||
| Autor: | Jonuscheit, Marc [Autor] | |||||||
| Dateien: |
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| Beitragende: | Prof. Dr. Heinzel, Thomas [Gutachter] Prof. Roden, Michael [Gutachter] | |||||||
| Stichwörter: | 31P, MRS, liver, metabolism, adenosine triphosphate, inorganic phosphate, ATP synthesis | |||||||
| Dewey Dezimal-Klassifikation: | 500 Naturwissenschaften und Mathematik » 570 Biowissenschaften; Biologie | |||||||
| Beschreibung: | The liver plays a central role in managing the body’s energy balance, particularly in glucose and lipid metabolism. Adenosine triphosphate (ATP), the cell’s main energy molecule, is predominantly synthesized in hepatic mitochondria and is crucial for numerous biochemical reactions. Alterations in hepatic ATP levels are linked to diseases such as metabolic dysfunction-associated steatotic liver disease (MASLD) and diabetes mellitus, underscoring the need of an accurate non-invasive way to measure hepatic energy metabolism.
The doctoral thesis addresses this need by applying advanced clinical magnetic resonance (MR) technology. Particularly, new coil designs and specialized phosphorus-31 (31P) magnetic resonance spectroscopy (MRS) protocols were developed for non-invasive detection of phosphorus-containing metabolites, with a special focus on ATP and inorganic phosphate (Pi), in the human liver. In the course of this, an MRS-protocol for the absolute quantification of these metabolites using the phantom replacement technique was developed and validated. Furthermore, the reproducibility of the method was tested, and the obtained results were compared between the two applied MR surface coils. The findings showed that both coils delivered reliable and reproducible results, proving the method to be independent of the coil in use. Building on this foundation, a second study introduced a new 31P-MRS-approach to assess hepatic ATP synthesis rates, using the saturation transfer (ST) MRS technique. This technique was optimized by both in vitro and in vivo measurements, and subsequently applied to cohorts of healthy volunteers and people with type 1 diabetes (T1D). The results in the healthy cohort indicated that the method is reproducible, and furthermore, it was possible to reliably pick up clinically relevant differences in ATP synthesis between healthy individuals and people with T1D. In summary, the studies presented in this thesis demonstrate the feasibility of non-invasive and reproducible measurements of both hepatic 31P-metabolite concentrations and ATP synthesis rates in humans using a clinical MR scanner. These methods provide new possibilities for understanding hepatic metabolism, improving the diagnosis of metabolic diseases, and monitoring therapeutic interventions, representing promising tools for future clinical research applications. | |||||||
| Lizenz: |  Dieses Werk ist lizenziert unter einer Creative Commons Namensnennung 4.0 International Lizenz | |||||||
| Fachbereich / Einrichtung: | Mathematisch- Naturwissenschaftliche Fakultät | |||||||
| Dokument erstellt am: | 29.01.2026 | |||||||
| Dateien geändert am: | 29.01.2026 | |||||||
| Promotionsantrag am: | 16.07.2025 | |||||||
| Datum der Promotion: | 17.12.2025 |
