Dokument: Altered Gap Junction Network Topography in Mouse Models for Human Hereditary Deafness
| Titel: | Altered Gap Junction Network Topography in Mouse Models for Human Hereditary Deafness | |||||||
| URL für Lesezeichen: | https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=71558 | |||||||
| URN (NBN): | urn:nbn:de:hbz:061-20251128-122803-7 | |||||||
| Kollektion: | Publikationen | |||||||
| Sprache: | Englisch | |||||||
| Dokumententyp: | Wissenschaftliche Texte » Artikel, Aufsatz | |||||||
| Medientyp: | Text | |||||||
| Autoren: | Eitelmann, Sara [Autor] Petersilie, Laura [Autor] Rose, Christine R. [Autor] Stephan, Jonathan [Autor] | |||||||
| Dateien: |
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| Stichwörter: | otoferlin , spontaneous activity , astrocytes , gap junctions , deafness , auditory brainstem , voltage-activated calcium channel 1.3 , lateral superior olive | |||||||
| Beschreibung: | Anisotropic gap junctional coupling is a distinct feature of astrocytes in many brain regions. In the lateral superior olive (LSO), astrocytic networks are anisotropic and oriented orthogonally to the tonotopic axis. In CaV1.3 knock-out (KO) and otoferlin KO mice, where auditory brainstem nuclei are deprived from spontaneous cochlea-driven neuronal activity, neuronal circuitry is disturbed. So far it was unknown if this disturbance is also accompanied by an impaired topography of LSO astrocyte networks. To answer this question, we immunohistochemically analyzed the expression of astrocytic connexin (Cx) 43 and Cx30 in auditory brainstem nuclei. Furthermore, we loaded LSO astrocytes with the gap junction-permeable tracer neurobiotin and assessed the network shape and orientation. We found a strong elevation of Cx30 immunoreactivity in the LSO of CaV1.3 KO mice, while Cx43 levels were only slightly increased. In otoferlin KO mice, LSO showed a slight increase in Cx43 as well, whereas Cx30 levels were unchanged. The total number of tracer-coupled cells was unaltered and most networks were anisotropic in both KO strains. In contrast to the WTs, however, LSO networks were predominantly oriented parallel to the tonotopic axis and not orthogonal to it. Taken together, our data demonstrate that spontaneous cochlea-driven neuronal activity is not required per se for the formation of anisotropic LSO astrocyte networks. However, neuronal activity is required to establish the proper orientation of networks. Proper formation of LSO astrocyte networks thus necessitates neuronal input from the periphery, indicating a critical role of neuron-glia interaction during early postnatal development in the auditory brainstem. | |||||||
| Rechtliche Vermerke: | Originalveröffentlichung:
Eitelmann, S., Petersilie, L., Rose, C. R., & Stephan, J. (2020). Altered Gap Junction Network Topography in Mouse Models for Human Hereditary Deafness [OnlineRessource]. International Journal of Molecular Sciences, 21(19), Article 7376. https://doi.org/10.3390/ijms21197376 | |||||||
| Lizenz: |  Dieses Werk ist lizenziert unter einer Creative Commons Namensnennung 4.0 International Lizenz | |||||||
| Fachbereich / Einrichtung: | Mathematisch- Naturwissenschaftliche Fakultät | |||||||
| Dokument erstellt am: | 28.11.2025 | |||||||
| Dateien geändert am: | 28.11.2025 |
