Dokument: Platelets Boost Recruitment of CD133⁺ Bone Marrow Stem Cells to Endothelium and the Rodent Liver—The Role of P-Selectin/PSGL-1 Interactions
| Titel: | Platelets Boost Recruitment of CD133⁺ Bone Marrow Stem Cells to Endothelium and the Rodent Liver—The Role of P-Selectin/PSGL-1 Interactions | |||||||
| URL für Lesezeichen: | https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=71521 | |||||||
| URN (NBN): | urn:nbn:de:hbz:061-20251124-123734-0 | |||||||
| Kollektion: | Publikationen | |||||||
| Sprache: | Englisch | |||||||
| Dokumententyp: | Wissenschaftliche Texte » Artikel, Aufsatz | |||||||
| Medientyp: | Text | |||||||
| Autoren: | Lehwald-Tywuschik, Nadja [Autor] Duhme, Constanze [Autor] Pinchuk, Iryna [Autor] Kirchner, Julian [Autor] Homey, Bernhard [Autor] Bode, Johannes [Autor] Kubitz, Ralf [Autor] Knoefel, Wolfram T. [Autor] Schulte am Esch, Jan [Autor] Wieferich, Kristina [Autor] | |||||||
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| Stichwörter: | bone marrow stem cell , endothelial cells , platelets , CD133 , P-selectin , liver regeneration | |||||||
| Beschreibung: | We previously demonstrated that clinical administration of mobilized CD133+ bone marrow stem cells (BMSC) accelerates hepatic regeneration. Here, we investigated the potential of platelets to modulate CD133+BMSC homing to hepatic endothelial cells and sequestration to warm ischemic livers. Modulatory effects of platelets on the adhesion of CD133+BMSC to human and mouse liver-sinusoidal- and micro- endothelial cells (EC) respectively were evaluated in in vitro co-culture systems. CD133+BMSC adhesion to all types of EC were increased in the presence of platelets under shear stress. This platelet effect was mostly diminished by antagonization of P-selectin and its ligand P-Selectin-Glyco-Ligand-1 (PSGL-1). Inhibition of PECAM-1 as well as SDF-1 receptor CXCR4 had no such effect. In a model of the isolated reperfused rat liver subsequent to warm ischemia, the co-infusion of platelets augmented CD133+BMSC homing to the injured liver with heightened transmigration towards the extra sinusoidal space when compared to perfusion conditions without platelets. Extravascular co-localization of CD133+BMSC with hepatocytes was confirmed by confocal microscopy. We demonstrated an enhancing effect of platelets on CD133+BMSC homing to and transmigrating along hepatic EC putatively depending on PSGL-1 and P-selectin. Our insights suggest a new mechanism of platelets to augment stem cell dependent hepatic repair. | |||||||
| Rechtliche Vermerke: | Originalveröffentlichung:
Lehwald-Tywuschik, N., Duhme, C., Pinchuk, I., Kirchner, J. P., Wieferich, K., Schmelzle, M., Jurk, K., Windmöller, B. A., Hübner, W., Homey, B., Bode, J. G., Kubitz, R., Benhidjeb, T., Krüger, M., Robson, S. C., Knoefel, W. T., Kehrel, B. E., & Schulte am Esch, J. (2020). Platelets Boost Recruitment of CD133+ Bone Marrow Stem Cells to Endothelium and the Rodent Liver—The Role of P-Selectin/PSGL-1 Interactions [OnlineRessource]. International Journal of Molecular Sciences, 21(17), Article 6431. https://doi.org/10.3390/ijms21176431 | |||||||
| Lizenz: |  Dieses Werk ist lizenziert unter einer Creative Commons Namensnennung 4.0 International Lizenz | |||||||
| Fachbereich / Einrichtung: | Medizinische Fakultät | |||||||
| Dokument erstellt am: | 24.11.2025 | |||||||
| Dateien geändert am: | 24.11.2025 |
