Dokument: Ultraplexing: increasing the efficiency of long-read sequencing for hybrid assembly with k-mer-based multiplexing
| Titel: | Ultraplexing: increasing the efficiency of long-read sequencing for hybrid assembly with k-mer-based multiplexing | |||||||
| URL für Lesezeichen: | https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=71361 | |||||||
| URN (NBN): | urn:nbn:de:hbz:061-20251114-121319-4 | |||||||
| Kollektion: | Publikationen | |||||||
| Sprache: | Englisch | |||||||
| Dokumententyp: | Wissenschaftliche Texte » Artikel, Aufsatz | |||||||
| Medientyp: | Text | |||||||
| Autoren: | Dilthey, Alexander [Autor] Kaasch, Achim [Autor] Meyer, Sebastian A. [Autor] | |||||||
| Dateien: |
| |||||||
| Stichwörter: | k-mer , Multiplexing , Assembly graph , Genome assembly , Hybrid assembly , Bacterial genomics , Barcoding | |||||||
| Beschreibung: | Hybrid genome assembly has emerged as an important technique in bacterial genomics, but cost and labor requirements limit large-scale application. We present Ultraplexing, a method to improve per-sample sequencing cost and hands-on time of Nanopore sequencing for hybrid assembly by at least 50% compared to molecular barcoding while maintaining high assembly quality. Ultraplexing requires the availability of Illumina data and uses inter-sample genetic variability to assign reads to isolates, which obviates the need for molecular barcoding. Thus, Ultraplexing can enable significant sequencing and labor cost reductions in large-scale bacterial genome projects. | |||||||
| Rechtliche Vermerke: | Originalveröffentlichung:
Dilthey, A., Meyer, S. A., & Kaasch, A. (2020). Ultraplexing: increasing the efficiency of long-read sequencing for hybrid assembly with k-mer-based multiplexing. Genome Biology, 21, Article 68. https://doi.org/10.1186/s13059-020-01974-9 | |||||||
| Lizenz: |  Dieses Werk ist lizenziert unter einer Creative Commons Namensnennung 4.0 International Lizenz | |||||||
| Fachbereich / Einrichtung: | Mathematisch- Naturwissenschaftliche Fakultät | |||||||
| Dokument erstellt am: | 14.11.2025 | |||||||
| Dateien geändert am: | 14.11.2025 |
