Dokument: Generation of a Bartter syndrome type 3 patient-derived induced pluripotent stem cell line ISRM-BS3-UM18-iPSC (HHUUKDi014-A)
| Titel: | Generation of a Bartter syndrome type 3 patient-derived induced pluripotent stem cell line ISRM-BS3-UM18-iPSC (HHUUKDi014-A) | |||||||
| URL für Lesezeichen: | https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=70217 | |||||||
| URN (NBN): | urn:nbn:de:hbz:061-20250716-142343-1 | |||||||
| Kollektion: | Publikationen | |||||||
| Sprache: | Englisch | |||||||
| Dokumententyp: | Wissenschaftliche Texte » Artikel, Aufsatz | |||||||
| Medientyp: | Text | |||||||
| Autoren: | Thimm, Chantelle [Autor] Zhong, Chutong [Autor] Wruck, Wasco [Autor] Grillo, Alessandra [Autor] Mack, Rosanne [Autor] Bohndorf, Martina [Autor] Graffmann, Nina [Autor] Tang, Anson [Autor] D’Ambrosio, Viola [Autor] Wan, Elizabeth R. [Autor] | |||||||
| Dateien: |
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| Beschreibung: | SIX2-positive urine-derived renal progenitor cells (UdRPCs) were isolated from an 18-year-old Bartter syndrome type 3 (BS3) patient within a homozygous CLCNKB gene deletion. Two episomal-based plasmids expressing OCT4, SOX2, NANOG, KLF4, c-MYC and LIN28 we were able to generate an integration-free induced pluripotent stem cell line (iPSC). Pluripotency was confirmed by fluorescence-activated cell sorting analysis and immunocytochemistry for the markers-OCT4, SOX2, NANOG, TRA-1-60, TRA-1-81 and SSEA4. Embryoid body-based differentiation into the three germ layers was the conducted and confirmed by immunocytochemistry. Pluritest analysis revealed a Pearson correlation of 0,93. Short tandem repeat DNA fingerprinting and karyotype analyses were performed. | |||||||
| Rechtliche Vermerke: | Originalveröffentlichung:
Thimm, C., Zhong, C., Wruck, W., Grillo, A., Mack, R., Bohndorf, M., Graffmann, N., Tang, A., D’Ambrosio, V., Wan, E. R., Siew, K., Evans, R. D., Walsh, S. B., & Adjaye, J. (2025). Generation of a Bartter syndrome type 3 patient-derived induced pluripotent stem cell line ISRM-BS3-UM18-iPSC (HHUUKDi014-A). Stem Cell Research, 87, Article 103760. https://doi.org/10.1016/j.scr.2025.103760 | |||||||
| Lizenz: |  Dieses Werk ist lizenziert unter einer Creative Commons Namensnennung 4.0 International Lizenz | |||||||
| Fachbereich / Einrichtung: | Medizinische Fakultät | |||||||
| Dokument erstellt am: | 16.07.2025 | |||||||
| Dateien geändert am: | 16.07.2025 |
