Dokument: 31P-MRS saturation transfer for assessing human hepatic ATP synthesis at clinical field strength
| Titel: | 31P-MRS saturation transfer for assessing human hepatic ATP synthesis at clinical field strength | |||||||
| URL für Lesezeichen: | https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=69728 | |||||||
| URN (NBN): | urn:nbn:de:hbz:061-20250526-093010-0 | |||||||
| Kollektion: | Publikationen | |||||||
| Sprache: | Englisch | |||||||
| Dokumententyp: | Wissenschaftliche Texte » Artikel, Aufsatz | |||||||
| Medientyp: | Text | |||||||
| Autoren: | Jonuscheit, Marc [Autor] Korzekwa, Benedict [Autor] Schär, Michael [Autor] Mevenkamp, Julian [Autor] Wierichs, Stefan [Autor] Bobrov, Pavel [Autor] Sarabhai, Theresia [Autor] Kahl, Sabine [Autor] Roden, Michael [Autor] Schrauwen-Hinderling, Vera B. [Autor] | |||||||
| Dateien: |
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| Stichwörter: | Diabetes mellitus (type 1), Energy metabolism, Liver, Magnetic resonance spectroscopy, Adenosine triphosphate | |||||||
| Beschreibung: | Background
31P-magnetic resonance spectroscopy (MRS) saturation transfer (ST) allows for noninvasive investigation of liver energy metabolism by assessing flux rates of adenosine triphosphate (ATP) synthesis. However, this technique has rarely been applied at clinical field strengths because of long examination times and contamination from muscle tissue. Our aim was to establish a new method to robustly assess ATP synthesis using a clinical scanner. Methods A prospective single-center study was performed (January 2023–August 2024) within the German Diabetes Study. We established a suitable 31P-MRS ST protocol, tested it in vitro and in vivo and assessed its reproducibility. We assessed the hepatic apparent spin-lattice relaxation time of inorganic phosphate (T′1;Pi), equilibrium forward rate constant (kf ), and forward ATP synthesis rate (FATP) in nine control volunteers (CON) (six females) and eight patients (five females) with type 1 diabetes (T1D) and compared differences by ANOVA. Results Reproducibility assessment in nine CON, aged 27 ± 4 years (mean ± standard deviation), yielded coefficients of variation for repeated measurements of 7.1% and 21.3% for T′ 1;Pi and kf , respectively. Group comparison revealed higher hepatic kf (0.34 ± 0.03 s-1 versus 0.16 ± 0.03 s-1; p = 0.001) and FATP (35.3 ± 3.5 mM/min versus 16.4 ± 3.5 mM/min; p = 0.002) in CON than in T1D, aged 42 ± 15 years, respectively. Conclusion This 31P-MRS ST method allowed for robust assessment of hepatic ATP synthesis at clinical field strength and was sensitive enough to detect differences between CON and T1D volunteers. Relevance statement Noninvasive methods to investigate hepatic energy metabolism are urgently needed to evaluate liver health while preventing unnecessary biopsies. For broad clinical applicability, the robustness shown by the proposed method at clinical field strength is crucial. | |||||||
| Rechtliche Vermerke: | Originalveröffentlichung:
Jonuscheit, M., Korzekwa, B., Schär, M., Mevenkamp, J., Wierichs, S., Bobrov, P., Sarabhai, T. C., Kahl, S., Roden, M., & Schrauwen-Hinderling, V. (2025). 31P-MRS saturation transfer for assessing human hepatic ATP synthesis at clinical field strength. European Radiology Experimental, 9, Article 51. https://doi.org/10.1186/s41747-025-00588-9 | |||||||
| Lizenz: |  Dieses Werk ist lizenziert unter einer Creative Commons Namensnennung 4.0 International Lizenz | |||||||
| Fachbereich / Einrichtung: | Medizinische Fakultät | |||||||
| Dokument erstellt am: | 26.05.2025 | |||||||
| Dateien geändert am: | 26.05.2025 |
