Dokument: A modified surgical approach to induce circle Willis perforation in mice using the common carotid artery

Titel:A modified surgical approach to induce circle Willis perforation in mice using the common carotid artery
URL für Lesezeichen:https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=69598
URN (NBN):urn:nbn:de:hbz:061-20250507-131506-8
Kollektion:Publikationen
Sprache:Englisch
Dokumententyp:Wissenschaftliche Texte » Artikel, Aufsatz
Medientyp:Text
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Dateien vom 07.05.2025 / geändert 07.05.2025
Stichwörter:Needle puncture, Translational stroke research, Circle Willis perforation, Common carotid artery approach, Subarachnoid hemorrhage mouse model
Beschreibung:The Circle of Willis perforation (cWp) mouse model is widely used in subarachnoid hemorrhage (SAH) research but involves sacrificing the external carotid artery (ECA), which may have a potential affect on the hemodynamic in the carotid arteries and cortical perfusion. We propose a modified approach using needle puncture via the common carotid artery (CCA) to preserve carotid vascular integrity. Twenty-seven C57BL/6 mice were randomly assigned into three groups and underwent cWp surgery in three procedures: sham (n = 3), ECA (n = 12), and CCA (n = 12). Surgical duration, success rate, intraoperative intracranial pressure (ICP) fluctuations, 24-hour mortality, and neurological deficits were assessed. The CCA approach achieved a 100% success rate and shorter surgical duration than the ECA approach (ECA 73 ± 18 vs. CCA 36 ± 10 min, P < 0.05). ICP fluctuations and mortality rates were comparable between the ECA and CCA groups (P > 0.05), indicating that the CCA approach shared a similar pattern with the ECA approach. Neurological outcomes were similar across SAH groups (CCA SAH induction and ECA SAH induction) but worse than sham (P < 0.05) in terms of body weight loss, open-filed test and Rotarod test performances. This modified cWp CCA approach, which preserves the carotid structures, helps eliminate hemodynamic bias and offers a potentially more efficient alternative with a shorter surgical duration compared to the classical ECA approach. It may prove to be a valuable option for broader application in SAH preclinical research.
Rechtliche Vermerke:Originalveröffentlichung:
Zhang, R., Khan, D., & Muhammad, S. (2025). A modified surgical approach to induce circle Willis perforation in mice using the common carotid artery. Scientific Reports, 15, Article 13769. https://doi.org/10.1038/s41598-025-97603-1
Lizenz:Creative Commons Lizenzvertrag
Dieses Werk ist lizenziert unter einer Creative Commons Namensnennung 4.0 International Lizenz
Fachbereich / Einrichtung:Medizinische Fakultät
Dokument erstellt am:07.05.2025
Dateien geändert am:07.05.2025
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