Dokument: Comparative analysis of adenosine 1 receptor expression and function in hippocampal and hypothalamic neurons
| Titel: | Comparative analysis of adenosine 1 receptor expression and function in hippocampal and hypothalamic neurons | |||||||
| URL für Lesezeichen: | https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=69249 | |||||||
| URN (NBN): | urn:nbn:de:hbz:061-20250407-110225-6 | |||||||
| Kollektion: | Publikationen | |||||||
| Sprache: | Englisch | |||||||
| Dokumententyp: | Wissenschaftliche Texte » Artikel, Aufsatz | |||||||
| Medientyp: | Text | |||||||
| Autoren: | Wegmann, Lea [Autor] Haas, Helmut L. [Autor] Sergeeva, Olga A. [Autor] | |||||||
| Dateien: |
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| Stichwörter: | Tuberomamillary nucleus, Adenosine receptor 1, Adenosine, Histaminergic neurons, Patch-clamp, Single-cell RT-PCR | |||||||
| Beschreibung: | Background
Adenosine, an ATP degradation product, is a sleep pressure factor. The adenosine 1 receptor (A1R) reports sleep need. Histaminergic neurons (HN) of the tuberomamillary nucleus (TMN) fire exclusively during wakefulness and promote arousal. All of them express GABAA receptors and are inhibited by GABA. Does adenosine contribute to their silencing? Subjects and treatment Responses to adenosine were studied in mouse brain slices and primary dissociated cultures. For HN identification single-cell (sc)RT-PCR, reporter protein and pharmacology were used. Hippocampal Dentate Gyrus granular layer cells (DGgc) were studied in parallel. Methods Firing frequency was recorded in patch-clamp configuration or by microelectrode arrays. A1R-expression was studied by scRT-PCR and semiquantitative PCR. Results Most DGgc were inhibited through A1R, detected with scRT-PCR in 7 out of 10 PDZd2-positive DGgc; all HN were A1R negative. One HN out of 25 was inhibited by adenosine. The A1R mRNA level in the hippocampus was 6 times higher than in the caudal (posterior) hypothalamus. Response to adenosine was weaker in hypothalamic compared to hippocampal cultures. Conclusions Most HN are not inhibited by adenosine. | |||||||
| Rechtliche Vermerke: | Originalveröffentlichung:
Wegmann, L., Haas, H. L., & Sergeeva, O. (2025). Comparative analysis of adenosine 1 receptor expression and function in hippocampal and hypothalamic neurons. Inflammation Research, 74(1), Article 11. https://doi.org/10.1007/s00011-024-01980-8 | |||||||
| Lizenz: |  Dieses Werk ist lizenziert unter einer Creative Commons Namensnennung 4.0 International Lizenz | |||||||
| Fachbereich / Einrichtung: | Medizinische Fakultät | |||||||
| Dokument erstellt am: | 07.04.2025 | |||||||
| Dateien geändert am: | 07.04.2025 |
