Dokument: A step forward to the optimized HlyA type 1 secretion system through directed evolution
| Titel: | A step forward to the optimized HlyA type 1 secretion system through directed evolution | |||||||
| URL für Lesezeichen: | https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=68739 | |||||||
| URN (NBN): | urn:nbn:de:hbz:061-20250221-092523-7 | |||||||
| Kollektion: | Publikationen | |||||||
| Sprache: | Englisch | |||||||
| Dokumententyp: | Wissenschaftliche Texte » Artikel, Aufsatz | |||||||
| Medientyp: | Text | |||||||
| Autoren: | Pourhassan Najafabadi, Zohreh [Autor] Cui, Haiyang [Autor] Muckhoff, Neele [Autor] Davari, Mehdi D. [Autor] Smits, Sander H.J. [Autor] Schwaneberg, Ulrich [Autor] Schmitt, Lutz [Autor] | |||||||
| Dateien: |
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| Stichwörter: | Gram-negative bacteria, KnowVolution, Protein engineering, Protein secretion, Directed evolution, Bacterial secretion system | |||||||
| Beschreibung: | Secretion of proteins into the extracellular space has great advantages for the production of recombinant proteins. Type 1 secretion systems (T1SS) are attractive candidates to be optimized for biotechnological applications, as they have a relatively simple architecture compared to other classes of secretion systems. A paradigm of T1SS is the hemolysin A type 1 secretion system (HlyA T1SS) from Escherichia coli harboring only three membrane proteins, which makes the plasmid-based expression of the system easy. Although for decades the HlyA T1SS has been successfully applied for secretion of a long list of heterologous proteins from different origins as well as peptides, but its utility at commercial scales is still limited mainly due to low secretion titers of the system. To address this drawback, we engineered the inner membrane complex of the system, consisting of HlyB and HlyD proteins, following KnowVolution strategy. The applied KnowVolution campaign in this study provided a novel HlyB variant containing four substitutions (T36L/F216W/S290C/V421I) with up to 2.5-fold improved secretion for two hydrolases, a lipase and a cutinase. | |||||||
| Rechtliche Vermerke: | Originalveröffentlichung:
Pourhassan Najafabadi, Z., Cui, H., Wolff, N., Davari, M. D., Smits, S. H. J., Schwaneberg, U., & Schmitt, L. (2023). A step forward to the optimized HlyA type 1 secretion system through directed evolution. Applied microbiology and biotechnology, 107(16), 5131–5143. https://doi.org/10.1007/s00253-023-12653-7 | |||||||
| Lizenz: |  Dieses Werk ist lizenziert unter einer Creative Commons Namensnennung 4.0 International Lizenz | |||||||
| Fachbereich / Einrichtung: | Mathematisch- Naturwissenschaftliche Fakultät | |||||||
| Dokument erstellt am: | 21.02.2025 | |||||||
| Dateien geändert am: | 21.02.2025 |
