Dokument: Spectroscopic view on the interaction between the psoralen derivative amotosalen and DNA
| Titel: | Spectroscopic view on the interaction between the psoralen derivative amotosalen and DNA | |||||||
| URL für Lesezeichen: | https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=68102 | |||||||
| URN (NBN): | urn:nbn:de:hbz:061-20250113-095636-3 | |||||||
| Kollektion: | Publikationen | |||||||
| Sprache: | Englisch | |||||||
| Dokumententyp: | Wissenschaftliche Texte » Artikel, Aufsatz | |||||||
| Medientyp: | Text | |||||||
| Autoren: | Rademacher, Michelle P. [Autor] Rohn, Tim [Autor] Haselbach, Wiebke [Autor] Ott, Anna Theresa [Autor] Bringmann, Peter W. [Autor] Glich, Peter [Autor] | |||||||
| Dateien: |
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| Stichwörter: | Time-resolved spectroscopy, Amotosalen, DNA intercalation, Photoaddition, Photoinduced electron transfer, Pathogen reduction technology | |||||||
| Beschreibung: | Psoralens are eponymous for PUVA (psoralen plus UV-A radiation) therapy, which inter alia can be used to treat various skin diseases. Based on the same underlying mechanism of action, the synthetic psoralen amotosalen (AMO) is utilized in the pathogen reduction technology of the INTERCEPT® Blood System to inactivate pathogens in plasma and platelet components. The photophysical behavior of AMO in the absence of DNA is remarkably similar to that of the recently studied psoralen 4′-aminomethyl-4,5′,8-trimethylpsoralen (AMT). By means of steady-state and time-resolved spectroscopy, intercalation and photochemistry of AMO and synthetic DNA were studied. AMO intercalates with a higher affinity into A,T-only DNA (KD = 8.9 × 10−5 M) than into G,C-only DNA (KD = 6.9 × 10−4 M). AMO covalently photobinds to A,T-only DNA with a reaction quantum yield of ΦR = 0.11. Like AMT, it does not photoreact following intercalation into G,C-only DNA. Femto- and nanosecond transient absorption spectroscopy reveals the characteristic pattern of photobinding to A,T-only DNA. For AMO and G,C-only DNA, signatures of a photoinduced electron transfer are recorded. | |||||||
| Rechtliche Vermerke: | Originalveröffentlichung:
Rademacher, M. P., Rohn, T., Haselbach, W., Ott, A. T., Bringmann, P. W., & Gilch, P. (2024). Spectroscopic view on the interaction between the psoralen derivative amotosalen and DNA. Photochemical & Photobiological Sciences, 23(4), 693–709. https://doi.org/10.1007/s43630-024-00545-2 | |||||||
| Lizenz: |  Dieses Werk ist lizenziert unter einer Creative Commons Namensnennung 4.0 International Lizenz | |||||||
| Fachbereich / Einrichtung: | Mathematisch- Naturwissenschaftliche Fakultät | |||||||
| Dokument erstellt am: | 13.01.2025 | |||||||
| Dateien geändert am: | 13.01.2025 |
