Dokument: Monocyte transcriptome signatures of inflammation and enhanced neutrophil recruitment characterize immunopathology in the blood of tuberculosis patients
| Titel: | Monocyte transcriptome signatures of inflammation and enhanced neutrophil recruitment characterize immunopathology in the blood of tuberculosis patients | |||||||
| URL für Lesezeichen: | https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=68076 | |||||||
| URN (NBN): | urn:nbn:de:hbz:061-20250109-122255-6 | |||||||
| Kollektion: | Publikationen | |||||||
| Sprache: | Englisch | |||||||
| Dokumententyp: | Wissenschaftliche Texte » Artikel, Aufsatz | |||||||
| Medientyp: | Text | |||||||
| Autoren: | Ahor, Hubert Senanu [Autor] Vivekanandan, Monika M. [Autor] Adankwah, Ernest [Autor] Minadzi, Difery [Autor] Acheampong, Isaac [Autor] Aniagyei, Wilfred [Autor] Yeboah, Augustine [Autor] Arthur, Joseph F. [Autor] Lamptey, Milicent [Autor] Abass, Mohammed K. [Autor] | |||||||
| Dateien: |
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| Stichwörter: | Tuberculosis, Monocytes, Plasma immunopathology, Neutrophil granulocytes | |||||||
| Beschreibung: | Tuberculosis (TB) is characterized by immunopathology in the blood and monocytes have been shown to be highly sensitive to plasma environment changes in TB patients. Here, we investigated TB plasma effects on ‘reference monocytes’ using RNA sequencing to characterize a potential immunomodulatory role of monocytes in TB. Candidate pathways induced by plasma samples from TB patients (n=99) compared to healthy controls (n=62) were analyzed for changes in signal transduction, phenotype and secreted cytokines by flow cytometry. Finally, potential implications were characterized in blood samples from corresponding patients and controls.
Reference monocytes treated with TB plasma showed an enrichment of pathways involved in inflammation and chemotaxis. Inflammatory cytokines were accompanied by enhanced phosphorylation of STAT molecules (i.e., STAT1/3/5), and strong positive correlations were detected for Interleukin (IL)-6 only in TB plasma-treated monocytes. Moreover, monocyte chemokine receptors (i.e., CCR-1, CCR-5) and pro-inflammatory chemokines (i.e., CXCL-1, CXCL-2, CXCL-8, G-CSF, CCL-2) that attract granulocytes and monocytes were significantly higher in TB plasma-treated monocytes. Notably, corresponding clinical samples also showed higher plasma levels for a subset of inflammatory cytokines/chemokines and, in particular, high IL-6 levels correlated positively with accumulation of neutrophil granulocytes in the blood of TB patients. Finally, monocytes from TB patients were characterized by increased chemokine receptor expression, higher proportions of a CCR-2+ subpopulation and aberrant high SOCS3 expression. These results suggest that monocytes may play a significant role in amplifying plasma immunopathology, leading to sustained mobilization and accumulation of neutrophil granulocytes and chronic inflammation in the blood of TB patients. | |||||||
| Rechtliche Vermerke: | Originalveröffentlichung:
Ahor, H. S., Vivekanandan, M. M., Adankwah, E., Minadzi, D., Acheampong, I., Aniagyei, W., Yeboah, A., Arthur, J. F., Lamptey, M., Abass, M. K., Kumbel, F., Osei-Yeboah, F., Gawusu, A., Petzsch, P., Köhrer, K., Debrah, L. B., Owusu, D. O., Debrah, A., Mayatepek, E., … Jacobsen, M. (2024). Monocyte transcriptome signatures of inflammation and enhanced neutrophil recruitment characterize immunopathology in the blood of tuberculosis patients. Journal of Infection, 89(6), Article 106359. https://doi.org/10.1016/j.jinf.2024.106359 [https://doi.org/10.1016/j.jinf.2024.106359] | |||||||
| Lizenz: |  Dieses Werk ist lizenziert unter einer Creative Commons Namensnennung 4.0 International Lizenz | |||||||
| Fachbereich / Einrichtung: | Medizinische Fakultät | |||||||
| Dokument erstellt am: | 09.01.2025 | |||||||
| Dateien geändert am: | 09.01.2025 |
