Dokument: Mobility of sodium ions in agarose gels probed through combined single- and triple-quantum NMR

Titel:Mobility of sodium ions in agarose gels probed through combined single- and triple-quantum NMR
URL für Lesezeichen:https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=68059
URN (NBN):urn:nbn:de:hbz:061-20250107-131404-3
Kollektion:Publikationen
Sprache:Englisch
Dokumententyp:Wissenschaftliche Texte » Artikel, Aufsatz
Medientyp:Text
Autoren: Nimerovsky, Evgeny [Autor]
Sieme, Daniel [Autor]
Rezaei-Ghaleh, Nasrollah [Autor]
Dateien:
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Dateien vom 07.01.2025 / geändert 07.01.2025
Stichwörter:quadrupolar NMR, Counterions, Hydrogels, Condensates, Phase separation
Beschreibung:Metal ions, including biologically prevalent sodium ions, can modulate electrostatic interactions frequently involved in the stability of condensed compartments in cells. Quantitative characterization of heterogeneous ion dynamics inside biomolecular condensates demands new experimental approaches. Here we develop a 23Na NMR relaxation-based integrative approach to probe dynamics of sodium ions inside agarose gels as a model system. We exploit the electric quadrupole moment of spin-3/2 23Na nuclei and, through combination of single-quantum and triple-quantum-filtered 23Na NMR relaxation methods, disentangle the relaxation contribution of different populations of sodium ions inside gels. Three populations of sodium ions are identified: a population with bi-exponential relaxation representing ions within the slow motion regime and two populations with mono-exponential relaxation but at different rates. Our study demonstrates the dynamical heterogeneity of sodium ions inside agarose gels and presents a new experimental approach for monitoring dynamics of sodium and other spin-3/2 ions (e.g. chloride) in condensed environments.
Rechtliche Vermerke:Originalveröffentlichung:
Nimerovsky, E., Sieme, D., & Rezaie Ghaleh, N. (2024). Mobility of sodium ions in agarose gels probed through combined single- and triple-quantum NMR. Methods, 288, 55–64. https://doi.org/10.1016/j.ymeth.2024.05.015
Lizenz:Creative Commons Lizenzvertrag
Dieses Werk ist lizenziert unter einer Creative Commons Namensnennung 4.0 International Lizenz
Fachbereich / Einrichtung:Mathematisch- Naturwissenschaftliche Fakultät
Dokument erstellt am:07.01.2025
Dateien geändert am:07.01.2025
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