Dokument: Understanding the dynamics of Plant - Bacteria - Bacteriophage interactions as a means to improve plant performance
| Titel: | Understanding the dynamics of Plant - Bacteria - Bacteriophage interactions as a means to improve plant performance | |||||||
| URL für Lesezeichen: | https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=68000 | |||||||
| URN (NBN): | urn:nbn:de:hbz:061-20250116-104438-2 | |||||||
| Kollektion: | Dissertationen | |||||||
| Sprache: | Englisch | |||||||
| Dokumententyp: | Wissenschaftliche Abschlussarbeiten » Dissertation | |||||||
| Medientyp: | Text | |||||||
| Autor: | Erdrich, Sebastian Hermann [Autor] | |||||||
| Dateien: |
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| Beitragende: | Prof. Dr. Schurr, Ulrich [Gutachter] Prof. Dr. Frunzke, Julia [Gutachter] | |||||||
| Stichwörter: | Bacteriophage, Plant biocontrol, Xanthomonas, seed coating | |||||||
| Dewey Dezimal-Klassifikation: | 500 Naturwissenschaften und Mathematik » 570 Biowissenschaften; Biologie | |||||||
| Beschreibung: | Plant protection is crucial in the context of a secure food supply. With antibiotic-resistant
bacteria on the rise, we explore new, sustainable plant protection strategies and utilise nat urally occurring bacterial viruses to counter pathogenic bacteria. These viruses, known as bacteriophages, are highly specific and outnumber bacteria by a factor of ten, and are present in every habitat on Earth. Despite their abundance, the number of available isolates for plant pathogenic bacteria is still very limited. The bacterial genus of Xanthomonas contains many well-known plant pathogens with the ability to infect some of the most important crop plants, causing significant economic damage. Unfortunately, classical pest control strategies are neither particularly efficient nor sustainable. Investigating phage-based strategies, we set the foundation in our lab by isolating seven novel Xanthomonas phages (Langgrundblatt1, Langgrundblatt2, Pfeifenkraut, Laurilin, Elanor, Mallos, and Seregon). As part of this PhD project, we further characterised, classified and tested them for their biocontrol potential in vitro. Besides good prerequisites for subsequent in planta experiments, we established four taxonomic novel genera. With seeds being one of the major transmission routes for bacterial pathogens in agriculture, we tested strategies to protect plants from the early stages. Therefore, phages for two im portant crop pathogens, Pseudomonas syringae and Agrobacterium fabrum (tumefaciens), were isolated and tested for their interaction with the seed coat mucilage, deepening the understanding of seed-based biocontrol. Some of the tested phages were highly dependent on mucilage for seed binding, whereas podophage Athelas showed the highest dependency. The significance of this observation was broadened by testing further podoviruses of the Autographiviridae family obtained from the systematic E. coli (BASEL) phage collection. These showed a similar dependence on the mucilage for seed adhesion. Phage coating effectively increased the survival rate of plant seedlings in the presence of the pathogen. Long-term activity tests revealed a high stability of phages on seed sur faces. The utilisation of non-virulent host strains was further successfully applied to enrich the presence of infectious phage particles on seed surfaces. Altogether, our study highlights the potential of phage-based applications as sustainable biocontrol strategy at the seed level. A further part of this work aimed at gaining a molecular understanding of the tripartite interaction between plants, bacteria, and phages in a novel tripartite transcriptomics ap proach. We aimed to fill the knowledge gap on how the plants gene expression is responding during phage-based biocontrol. For this purpose, a gnotobiotic system was used to study infection of Arabidopsis thaliana with the plant pathogen Xanthomonas campestris. Here, the application of the Xanthomonas phage Seregon could successfully counteract the bac terial infection almost to the level of the uninfected control. Additionally, we observed a significant variation in the expression of defence-related genes throughout the tripartite interaction. While X. campestris inoculation led to expression of several salicylic acid respon sive genes like WRKY70 and WAK1, the treatment of X. campestris with phage Seregon led to a significantly reduced upregulation of these genes. We also identified GRP3.1 as uniquely upregulated in response to phage-based control of X. campestris. In summary, this thesis offers unprecedented insights into the molecular-level tripartite interactions between plants, bacteria, and phages, thereby establishing a crucial foundation for the development of sustainable biocontrol strategies in agriculture utilizing phages. | |||||||
| Lizenz: |  Dieses Werk ist lizenziert unter einer Creative Commons Namensnennung 4.0 International Lizenz | |||||||
| Fachbereich / Einrichtung: | Mathematisch- Naturwissenschaftliche Fakultät | |||||||
| Dokument erstellt am: | 16.01.2025 | |||||||
| Dateien geändert am: | 16.01.2025 | |||||||
| Promotionsantrag am: | 26.06.2024 | |||||||
| Datum der Promotion: | 29.07.2024 |
