Dokument: GABARAP interacts with EGFR — supporting the unique role of this hAtg8 protein during receptor trafficking
| Titel: | GABARAP interacts with EGFR — supporting the unique role of this hAtg8 protein during receptor trafficking | |||||||
| URL für Lesezeichen: | https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=67976 | |||||||
| URN (NBN): | urn:nbn:de:hbz:061-20241213-111555-1 | |||||||
| Kollektion: | Publikationen | |||||||
| Sprache: | Englisch | |||||||
| Dokumententyp: | Wissenschaftliche Texte » Artikel, Aufsatz | |||||||
| Medientyp: | Text | |||||||
| Autoren: | Üffing, Alina [Autor] Weiergräber, Oliver H. [Autor] Schwarten, Melanie [Autor] Hoffmann, Silke [Autor] Willbold, Dieter [Autor] | |||||||
| Dateien: |
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| Stichwörter: | canonical binding, complex structure, surface receptor, Alphafold, X-ray, LC3- interacting region, NMR, paralog-specificity | |||||||
| Beschreibung: | The human Atg8 family member GABARAP is involved in numerous autophagy-related and -unrelated processes. We recently observed that specifically the deficiency of GABARAP enhances epidermal growth factor receptor (EGFR) degradation upon ligand stimulation. Here, we report on two putative LC3-interacting regions (LIRs) within EGFR, the first of which
(LIR1) is selected as a GABARAP binding site in silico. Indeed, in vitro interaction studies reveal preferential binding of LIR1 to GABARAP and GABARAPL1. Our X-ray data demonstrate interaction of core LIR1 residues FLPV with both hydrophobic pockets of GABARAP suggesting canonical binding. Although LIR1 occupies the LIR docking site, GABARAP Y49 and L50 appear dispensable in this case. Our data support the hypothesis that GABARAP affects the fate of EGFR at least in part through direct binding. | |||||||
| Rechtliche Vermerke: | Originalveröffentlichung:
Üffing, A., Weiergräber, O. H., Schwarten, M., Hoffmann, S., & Willbold, D. (2024). GABARAP interacts with EGFR — supporting the unique role of this hAtg8 protein during receptor trafficking. FEBS Letters / Federation of European Biochemical Societies, 598(21), 2656–2669. https://doi.org/10.1002/1873-3468.14997 | |||||||
| Lizenz: |  Dieses Werk ist lizenziert unter einer Creative Commons Namensnennung 4.0 International Lizenz | |||||||
| Fachbereich / Einrichtung: | Mathematisch- Naturwissenschaftliche Fakultät | |||||||
| Dokument erstellt am: | 13.12.2024 | |||||||
| Dateien geändert am: | 13.12.2024 |
