Dokument: Immunoadsorption as a method of antibody donation during the COVID‐19 pandemic
| Titel: | Immunoadsorption as a method of antibody donation during the COVID‐19 pandemic | |||||||
| URL für Lesezeichen: | https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=67964 | |||||||
| URN (NBN): | urn:nbn:de:hbz:061-20241212-120148-6 | |||||||
| Kollektion: | Publikationen | |||||||
| Sprache: | Englisch | |||||||
| Dokumententyp: | Wissenschaftliche Texte » Artikel, Aufsatz | |||||||
| Medientyp: | Text | |||||||
| Autoren: | Rothenburg, Jannik [Autor] Rink-Baron, Silke [Autor] Müller, Lisa [Autor] Ostermann, Philipp Niklas [Autor] Fischer, Johannes C. [Autor] Hermsen, Derik [Autor] Stegbauer, Johannes [Autor] Moldenhauer, Anja [Autor] | |||||||
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| Stichwörter: | antibody donation, immunoadsorption, COVID-19 convalescence, virus neutralization | |||||||
| Beschreibung: | Background and Objectives:
Initial therapeutic efforts to treat severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) included the use of plasma from convalescent donors containing anti-SARS-CoV-2 antibodies. High-neutralizing antibody titres are required for therapeutic efficacy. This study aims to show that immunoadsorption followed by tangential flow filtration can be used to obtain antibody concentrates with high-neutralizing capacities. Materials and Methods: Eligible donors (n = 10, five males and three females) underwent immunoadsorption using adsorber columns specific for human antibodies. Glycine-washed out eluates of 1.5 L volume were further concentrated by tangential flow filtration using 30 kDa ultrafiltration membranes. The same membranes were applied for diafiltrations to exchange residual glycine for 0.9% normal saline. Results: Antibody concentrates were obtained within 8 h from the start of donation and had 4.58 ± 1.95, 3.28 ± 1.28 and 2.02 ± 0.92 times higher total IgG, IgA and IgM concentrations, 3.29 ± 1.62 and 3.74 ± 0.6 times higher SARS-CoV-2 N and S antibody concentrations and 3.85 ± 1.71 times higher SARS-CoV-2 S-specific IgG concentrations compared to the donors’ peripheral blood. The specific SARS-CoV-2 virus neutralization capacities increased in all but one concentrate. All antibody concentrates (50–70 mL final volume) passed microbiological tests, were free of hazardous glycine levels and could be stored at minus 80 celsius and 4 celsius for 1 year with 20 ± 3% antibody loss. | |||||||
| Rechtliche Vermerke: | Originalveröffentlichung:
Rothenburg, J., Rink‐Baron, S., Müller, L., Ostermann, P., Fischer, J., Hermsen, D. F., Stegbauer, J., & Moldenhauer, A. (2024). Immunoadsorption as a method of antibody donation during the COVID‐19 pandemic. Vox Sanguinis, 119(8), 792–800. https://doi.org/10.1111/vox.13647 | |||||||
| Lizenz: |  Dieses Werk ist lizenziert unter einer Creative Commons Namensnennung 4.0 International Lizenz | |||||||
| Fachbereich / Einrichtung: | Medizinische Fakultät | |||||||
| Dokument erstellt am: | 12.12.2024 | |||||||
| Dateien geändert am: | 12.12.2024 |
