Dokument: Characterization of size-tuneable aescin-lipid nanoparticles as platform for stabilization of membrane proteins
| Titel: | Characterization of size-tuneable aescin-lipid nanoparticles as platform for stabilization of membrane proteins | |||||||
| URL für Lesezeichen: | https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=67648 | |||||||
| URN (NBN): | urn:nbn:de:hbz:061-20241122-100150-6 | |||||||
| Kollektion: | Publikationen | |||||||
| Sprache: | Englisch | |||||||
| Dokumententyp: | Wissenschaftliche Texte » Artikel, Aufsatz | |||||||
| Medientyp: | Text | |||||||
| Autoren: | Escobedo, Fatima [Autor] Gospalswamy, Mohanraj [Autor] Hägerbäumer, Pia [Autor] Stank, Tim Julian [Autor] Victor, Julian [Autor] Groth, Georg [Autor] Gohlke, Holger [Autor] Dargel, Carina [Autor] Hellweg, Thomas [Autor] Etzkorn, Manuel [Autor] | |||||||
| Dateien: |
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| Stichwörter: | aescin-DMPC-nanoparticles, nuclear magnetic resonance (NMR), molecular interaction, bacteriorhodopsin (BR) | |||||||
| Beschreibung: | Disc-like lipid nanoparticles stabilized by saponin biosurfactants display fascinating properties, including their temperature-driven re-organization. β-Aescin, a saponin from seed extract of the horse chestnut tree, shows strong interactions with lipid membranes and has gained interest due to its beneficial therapeutic implications as well as its ability to decompose continuous lipid membranes into size-tuneable discoidal nanoparticles. Here, we characterize lipid nanoparticles formed by aescin and the phospholipid 1,2-dimyristoyl-sn-glycero-3-phosphocholine. We present site-resolved insights into central molecular interactions and their modulations by temperature and aescin content. Using the membrane protein bacteriorhodopsin, we additionally demonstrate that, under defined conditions, aescin-lipid discs can accommodate medium-sized transmembrane proteins. Our data reveal the general capability of this fascinating system to generate size-tuneable aescin-lipid-protein particles, opening the road for further applications in biochemical, biophysical and structural studies. | |||||||
| Rechtliche Vermerke: | Originalveröffentlichung:
Escobedo González, F. C., Gopalswamy, M., Hägerbäumer, P., Stank, T. J., Victor, J., Groth, G., Gohlke, H., Dargel, C., Hellweg, T., & Etzkorn, M. (2024). Characterization of size-tuneable aescin-lipid nanoparticles as platform for stabilization of membrane proteins. Colloids and Surfaces / B B, Biointerfaces, 242, Article 114071. https://doi.org/10.1016/j.colsurfb.2024.114071 | |||||||
| Lizenz: |  Dieses Werk ist lizenziert unter einer Creative Commons Namensnennung 4.0 International Lizenz | |||||||
| Fachbereich / Einrichtung: | Mathematisch- Naturwissenschaftliche Fakultät | |||||||
| Dokument erstellt am: | 22.11.2024 | |||||||
| Dateien geändert am: | 22.11.2024 |
