Dokument: NERNST: a genetically-encoded ratiometric non-destructive sensing tool to estimate NADP(H) redox status in bacterial, plant and animal systems
| Titel: | NERNST: a genetically-encoded ratiometric non-destructive sensing tool to estimate NADP(H) redox status in bacterial, plant and animal systems | |||||||
| URL für Lesezeichen: | https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=67470 | |||||||
| URN (NBN): | urn:nbn:de:hbz:061-20241112-113957-1 | |||||||
| Kollektion: | Publikationen | |||||||
| Sprache: | Englisch | |||||||
| Dokumententyp: | Wissenschaftliche Texte » Artikel, Aufsatz | |||||||
| Medientyp: | Text | |||||||
| Autoren: | Molinari, Pamela E. [Autor] Krapp, Adriana R. [Autor] Weiner, Andrea [Autor] Beyer, Hannes M. [Autor] Kondadi, Arun Kumar [Autor] Blomeier, Tim [Autor] López, Melina [Autor] Bustos-Sanmamed,Pilar [Autor] Tevere, Evelyn [Autor] Weber, Wilfried [Autor] | |||||||
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| Beschreibung: | NADP(H) is a central metabolic hub providing reducing equivalents to multiple biosynthetic, regulatory and antioxidative pathways in all living organisms. While biosensors are available to determine NADP+ or NADPH levels in vivo, no probe exists to estimate the NADP(H) redox status, a determinant of the cell energy availability. We describe herein the design and characterization of a genetically-encoded ratiometric biosensor, termed NERNST, able to interact with NADP(H) and estimate ENADP(H). NERNST consists of a redox-sensitive green fluorescent protein (roGFP2) fused to an NADPH-thioredoxin reductase C module which selectively monitors NADP(H) redox states via oxido-reduction of the roGFP2 moiety. NERNST is functional in bacterial, plant and animal cells, and organelles such as chloroplasts and mitochondria. Using NERNST, we monitor NADP(H) dynamics during bacterial growth, environmental stresses in plants, metabolic challenges to mammalian cells, and wounding in zebrafish. NERNST estimates the NADP(H) redox poise in living organisms, with various potential applications in biochemical, biotechnological and biomedical research. | |||||||
| Rechtliche Vermerke: | Originalveröffentlichung:
Molinari, P. E., Krapp, A. R., Weiner, A., Beyer, H. M., Kondadi, A. K., Blomeier, T., López, M., Bustos-Sanmamed, P., Tevere, E., Weber, W., Reichert, A., Calcaterra, N. B., Beller, M., Carrillo, N., & Zurbriggen, M. (2023). NERNST: a genetically-encoded ratiometric non-destructive sensing tool to estimate NADP(H) redox status in bacterial, plant and animal systems. Nature Communications, 14, Article 3277. https://doi.org/10.1038/s41467-023-38739-4 | |||||||
| Lizenz: |  Dieses Werk ist lizenziert unter einer Creative Commons Namensnennung 4.0 International Lizenz | |||||||
| Fachbereich / Einrichtung: | Mathematisch- Naturwissenschaftliche Fakultät Medizinische Fakultät | |||||||
| Dokument erstellt am: | 12.11.2024 | |||||||
| Dateien geändert am: | 12.11.2024 |
