Dokument: Cristae dynamics is modulated in bioenergetically compromised mitochondria
| Titel: | Cristae dynamics is modulated in bioenergetically compromised mitochondria | |||||||
| URL für Lesezeichen: | https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=67265 | |||||||
| URN (NBN): | urn:nbn:de:hbz:061-20241029-144830-6 | |||||||
| Kollektion: | Publikationen | |||||||
| Sprache: | Englisch | |||||||
| Dokumententyp: | Wissenschaftliche Texte » Artikel, Aufsatz | |||||||
| Medientyp: | Text | |||||||
| Autoren: | Golombek, Mathias [Autor] Tsigaras, Thanos [Autor] Schaumkessel, Yulia [Autor] Hänsch, Sebastian [Autor] Weidtkamp-Peters, Stefanie [Autor] Anand, Ruchika [Autor] Reichert, Andreas S. [Autor] Kondadi, Arun Kumar [Autor] | |||||||
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| Beschreibung: | Cristae membranes have been recently shown to undergo intramitochondrial merging and splitting events. Yet, the metabolic and bioenergetic factors regulating them are unclear. Here, we investigated whether and how cristae morphology and dynamics are dependent on oxidative phosphorylation (OXPHOS) complexes, the mitochondrial membrane potential (ΔΨm), and the ADP/ATP nucleotide translocator. Advanced live-cell STED nanoscopy combined with in-depth quantification were employed to analyse cristae morphology and dynamics after treatment of mammalian cells with rotenone, antimycin A, oligomycin A, and CCCP. This led to formation of enlarged mitochondria along with reduced cristae density but did not impair cristae dynamics. CCCP treatment leading to ΔΨm abrogation even enhanced cristae dynamics showing its ΔΨm-independent nature. Inhibition of OXPHOS complexes was accompanied by reduced ATP levels but did not affect cristae dynamics. However, inhibition of ADP/ATP exchange led to aberrant cristae morphology and impaired cristae dynamics in a mitochondrial subset. In sum, we provide quantitative data of cristae membrane remodelling under different conditions supporting an important interplay between OXPHOS, metabolite exchange, and cristae membrane dynamics. | |||||||
| Rechtliche Vermerke: | Originalveröffentlichung:
Golombek, M., Tsigaras, T., Schaumkessel, Y., Hänsch, S., Weidtkamp-Peters, S., Anand, R., Reichert, A., & Kondadi, A. K. (2023). Cristae dynamics is modulated in bioenergetically compromised mitochondria. Life Science Alliance, 7(2), Article e202302386. https://doi.org/10.26508/lsa.202302386 | |||||||
| Lizenz: |  Dieses Werk ist lizenziert unter einer Creative Commons Namensnennung 4.0 International Lizenz | |||||||
| Fachbereich / Einrichtung: | Mathematisch- Naturwissenschaftliche Fakultät Medizinische Fakultät | |||||||
| Dokument erstellt am: | 29.10.2024 | |||||||
| Dateien geändert am: | 29.10.2024 |
