Dokument: The Impact of p70S6 Kinase-Dependent Phosphorylation of Gemin2 in UsnRNP Biogenesis
| Titel: | The Impact of p70S6 Kinase-Dependent Phosphorylation of Gemin2 in UsnRNP Biogenesis | |||||||
| URL für Lesezeichen: | https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=67254 | |||||||
| URN (NBN): | urn:nbn:de:hbz:061-20241029-111449-1 | |||||||
| Kollektion: | Publikationen | |||||||
| Sprache: | Englisch | |||||||
| Dokumententyp: | Wissenschaftliche Texte » Artikel, Aufsatz | |||||||
| Medientyp: | Text | |||||||
| Autoren: | Esser, Lea Marie [Autor] Li, Qiaoping [Autor] Jüdt, Maximilian [Autor] Kähne, Thilo [Autor] Stork, Björn [Autor] Grimmler, Matthias [Autor] Wesselborg, Sebastian [Autor] Peter, Christoph [Autor] | |||||||
| Dateien: |
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| Stichwörter: | UsnRNP biogenesis, mTOR pathway, Gemin2, p70S6K, post-translational modifications | |||||||
| Beschreibung: | The survival motor neuron (SMN) complex is a multi-megadalton complex involved in post-transcriptional gene expression in eukaryotes via promotion of the biogenesis of uridine-rich small nuclear ribonucleoproteins (UsnRNPs). The functional center of the complex is formed from the SMN/Gemin2 subunit. By binding the pentameric ring made up of the Sm proteins SmD1/D2/E/F/G and allowing for their transfer to a uridine-rich short nuclear RNA (UsnRNA), the Gemin2 protein in particular is crucial for the selectivity of the Sm core assembly. It is well established that post-translational modifications control UsnRNP biogenesis. In our work presented here, we emphasize the crucial role of Gemin2, showing that the phospho-status of Gemin2 influences the capacity of the SMN complex to condense in Cajal bodies (CBs) in vivo. Additionally, we define Gemin2 as a novel and particular binding partner and phosphorylation substrate of the mTOR pathway kinase ribosomal protein S6 kinase beta-1 (p70S6K). Experiments using size exclusion chromatography further demonstrated that the Gemin2 protein functions as a connecting element between the 6S complex and the SMN complex. As a result, p70S6K knockdown lowered the number of CBs, which in turn inhibited in vivo UsnRNP synthesis. In summary, these findings reveal a unique regulatory mechanism of UsnRNP biogenesis. | |||||||
| Rechtliche Vermerke: | Originalveröffentlichung:
Esser, L. M., Li, Q., Jüdt, M., Kähne, T., Stork, B., Grimmler, M., Wesselborg, S., & Peter, C. (2023). The Impact of p70S6 Kinase-Dependent Phosphorylation of Gemin2 in UsnRNP Biogenesis. International Journal of Molecular Sciences, 24(21), Article 15552. https://doi.org/10.3390/ijms242115552 | |||||||
| Lizenz: |  Dieses Werk ist lizenziert unter einer Creative Commons Namensnennung 4.0 International Lizenz | |||||||
| Fachbereich / Einrichtung: | Medizinische Fakultät | |||||||
| Dokument erstellt am: | 29.10.2024 | |||||||
| Dateien geändert am: | 29.10.2024 |
