Dokument: BCG-Vaccinated Children with Contact to Tuberculosis Patients Show Delayed Conversion of Mycobacterium tuberculosis-Specific IFN-γ Release
| Titel: | BCG-Vaccinated Children with Contact to Tuberculosis Patients Show Delayed Conversion of Mycobacterium tuberculosis-Specific IFN-γ Release | |||||||
| URL für Lesezeichen: | https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=67055 | |||||||
| URN (NBN): | urn:nbn:de:hbz:061-20241015-111010-4 | |||||||
| Kollektion: | Publikationen | |||||||
| Sprache: | Englisch | |||||||
| Dokumententyp: | Wissenschaftliche Texte » Artikel, Aufsatz | |||||||
| Medientyp: | Text | |||||||
| Autoren: | Owusu, Dorcas O. [Autor] Adankwah, Ernest [Autor] Aniagyei, Wilfred [Autor] Acheampong, Isaac [Autor] Minadzi, Difery [Autor] Yeboah, Augustine [Autor] Arthur, Joseph F. [Autor] Lamptey, Milicent [Autor] Vivekanandan, Monika M. [Autor] Abass, Mohammed K. [Autor] | |||||||
| Dateien: |
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| Stichwörter: | Mycobacterium tuberculosis infection, IFN-y, children, tuberculosis contact, BCG vaccination | |||||||
| Beschreibung: | Mycobacterium (M.) bovis BCG vaccination is recommended for healthy babies after birth in several countries with a high prevalence of tuberculosis, including Ghana. Previous studies showed that BCG vaccination prevents individuals from developing severe clinical manifestations of tuberculosis, but BCG vaccination effects on the induction of IFN-γ after M. tuberculosis infection have hardly been investigated. Here, we performed IFN-γ-based T-cell assays (i.e., IFN-γ Release Assay, IGRA; T-cell activation and maturation marker assay, TAM-TB) in children who had contact with index tuberculosis patients (contacts). These contacts were classified as either being BCG vaccinated at birth (n = 77) or non-BCG-vaccinated (n = 17) and were followed up at three timepoints for a period of one year to determine immune conversion after M. tuberculosis exposure and potential infection. At baseline and month 3, BCG-vaccinated contacts had significantly lower IFN-γ levels after stimulation with M. tuberculosis-specific proteins as compared to non-BCG-vaccinated contacts. This resulted in decreased proportions of positive IGRA results (BCG-vaccinated: 60% at baseline, 57% at month 3; non-BCG-vaccinated: 77% and 88%, respectively) at month 3. However, until month 12, immune conversion in BCG-vaccinated contacts led to balanced proportions in IGRA responders and IFN-γ expression between the study groups. TAM-TB assay analyses confirmed higher proportions of IFN-γ-positive T-cells in non-BCG-vaccinated contacts. Low proportions of CD38-positive M. tuberculosis-specific T-cells were only detected in non-BCG-vaccinated contacts at baseline. These results suggest that BCG vaccination causes delayed immune conversion as well as differences in the phenotype of M. tuberculosis-specific T-cells in BCG-vaccinated contacts of tuberculosis patients. These differences are immune biomarker candidates for protection against the development of severe clinical tuberculosis manifestations. | |||||||
| Rechtliche Vermerke: | Originalveröffentlichung:
Owusu, D. O., Adankwah, E., Aniagyei, W., Acheampong, I., Minadzi, D., Yeboah, A., Arthur, J. F., Lamptey, M., Vivekanandan, M. M., Abass, M. K., Kumbel, F., Osei-Yeboah, F., Gawusu, A., Batsa Debrah, L., Debrah, A., Mayatepek, E., Seyfarth, J., Phillips, R. O., & Jacobsen, M. (2023). BCG-Vaccinated Children with Contact to Tuberculosis Patients Show Delayed Conversion of Mycobacterium tuberculosis-Specific IFN-γ Release [OnlineRessource]. Vaccines, 11(4), Article 855. https://doi.org/10.3390/vaccines11040855 | |||||||
| Lizenz: |  Dieses Werk ist lizenziert unter einer Creative Commons Namensnennung 4.0 International Lizenz | |||||||
| Fachbereich / Einrichtung: | Medizinische Fakultät | |||||||
| Dokument erstellt am: | 15.10.2024 | |||||||
| Dateien geändert am: | 19.11.2024 |
