Dokument: Influence of Storage Conditions on Decellularized Porcine Conjunctiva
| Titel: | Influence of Storage Conditions on Decellularized Porcine Conjunctiva | |||||||
| URL für Lesezeichen: | https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=67003 | |||||||
| URN (NBN): | urn:nbn:de:hbz:061-20241010-113407-7 | |||||||
| Kollektion: | Publikationen | |||||||
| Sprache: | Englisch | |||||||
| Dokumententyp: | Wissenschaftliche Texte » Artikel, Aufsatz | |||||||
| Medientyp: | Text | |||||||
| Autoren: | Skornia, Adam [Autor] Geerling, Gerd [Autor] Spaniol, Kristina [Autor] Witt, Joana [Autor] | |||||||
| Dateien: |
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| Stichwörter: | scaffold, decellularized conjunctiva, conjunctival reconstruction, decellularization, human conjunctival epithelial cell | |||||||
| Beschreibung: | Porcine decellularized conjunctiva (PDC) represents a promising alternative source for conjunctival reconstruction. Methods of its re-epithelialization in vitro with primary human conjunctival epithelial cells (HCEC) have already been established. However, a long-term storage method is required for a simplified clinical use of PDC. This study investigates the influence of several storage variants on PDC. PDC were stored in (1) phosphate-buffered saline solution (PBS) at 4 °C, (2) in glycerol-containing epithelial cell medium (EM/gly) at −80 °C and (3) in dimethyl sulfoxide-containing epithelial cell medium (EM/DMSO) at −196 °C in liquid nitrogen for two and six months, respectively. Fresh PDC served as control. Histological structure, biomechanical parameters, the content of collagen and elastin and the potential of re-epithelialization with primary HCEC under cultivation for 14 days were compared (n = 4–10). In all groups, PDC showed a well-preserved extracellular matrix without structural disruptions and with comparable fiber density (p ≥ 0.74). Collagen and elastin content were not significantly different between the groups (p ≥ 0.18; p ≥ 0.13, respectively). With the exception of the significantly reduced tensile strength of PDC after storage at −196 °C in EM/DMSO for six months (0.46 ± 0.21 MPa, p = 0.02), no differences were seen regarding the elastic modulus, tensile strength and extensibility compared to control (0.87 ± 0.25 MPa; p ≥ 0.06). The mean values of the epithelialized PDC surface ranged from 51.9 ± 8.8% (−196 °C) to 78.3 ± 4.4% (−80 °C) and did not differ significantly (p ≥ 0.35). In conclusion, all examined storage methods were suitable for storing PDC for at least six months. All PDC were able to re-epithelialize, which rules out cytotoxic influences of the storage conditions and suggests preserved biocompatibility for in vivo application. | |||||||
| Rechtliche Vermerke: | Originalveröffentlichung:
Skornia, A., Geerling, Gerd , Spaniol, K., & Witt, J. (2023). Influence of Storage Conditions on Decellularized Porcine Conjunctiva [OnlineRessource]. Bioengineering, 10(3), Article 350. https://doi.org/10.3390/bioengineering10030350 | |||||||
| Lizenz: |  Dieses Werk ist lizenziert unter einer Creative Commons Namensnennung 4.0 International Lizenz | |||||||
| Fachbereich / Einrichtung: | Medizinische Fakultät | |||||||
| Dokument erstellt am: | 10.10.2024 | |||||||
| Dateien geändert am: | 16.10.2024 |
