Dokument: Establishment of novel model systems for synthetic microbial communities
| Titel: | Establishment of novel model systems for synthetic microbial communities | |||||||
| Weiterer Titel: | Etablierung neuer Modellsysteme für synthetische Konsortien | |||||||
| URL für Lesezeichen: | https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=66889 | |||||||
| URN (NBN): | urn:nbn:de:hbz:061-20241007-105125-4 | |||||||
| Kollektion: | Dissertationen | |||||||
| Sprache: | Englisch | |||||||
| Dokumententyp: | Wissenschaftliche Abschlussarbeiten » Dissertation | |||||||
| Medientyp: | Text | |||||||
| Autor: | Mager, Maurice [Autor] | |||||||
| Dateien: |
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| Beitragende: | Jun.-Prof. Dr. Axmann, Ilka M. [Gutachter] Prof. Dr. Fraune, Sebastian [Gutachter] | |||||||
| Stichwörter: | Microbiome, Vibrio natriegens, Curvibacter, Cyanobacteria | |||||||
| Dewey Dezimal-Klassifikation: | 500 Naturwissenschaften und Mathematik » 570 Biowissenschaften; Biologie | |||||||
| Beschreibungen: | Während der Großteil mikrobieller Forschung auf der Untersuchung einzelner, isolierter
Spezies beruht, ist die Erforschung von mikrobiellen Konsortien erst seit kurzem im Fokus. Bakterien koexistieren jedoch in ihrer natürlichen Umgebung hauptsächlich als Teil von solchen Konsortien, die von der Tiefsee bis zu heißen Quellen, von der Haut bis zum menschlichen Darm überall gefunden werden können. Diese Gemeinschaven sind an essentiellen geo- und biochemischen Transformationsprozessen beteiligt. Änderungen in ihren Zusammensetzungen konnten bei Menschen schon mit Krankheiten wie Alzheimer in Verbindung gebracht werden. Jedoch sind die fundamentalen Prinzipien und Dynamiken solcher mikrobiellen Konsortien kaum verstanden. Um eine einzelne Spezies tiefgehend zu verstehen, muss diese im Kontext ihrer Interaktionen mit ihrer Umgebung inklusive anderer Bakterien untersucht werden. Ziel dieser Arbeit ist die Weiterentwicklung bestehender und die Entwicklung neuer Modellsysteme zur Erforschung mikrobieller Konsortien. Im ersten Projekt wurde die Domestizierung von Curvibacter AEP1-3, einem natürlichen Bewohner von Hydra vulgaris, durch die Entwicklung neuer Genexpressionsysteme vorangetrieben. Mehrere neue Systeme wurden entdeckt und deren Genaktivität während verschiedener Wachstumsphasen gemessen. In einem weiteren Projekt wurde die Reproduzierbarkeit von Forschung mit Cyanobakterien anhand einer Genexpressionsstudie ermitelt, die in mehreren teilnehmenden Laboren in Europa durchgeführt wurde. Ziel war es, auf die mangelnde Reproduzierbarkeit von Ergebnissen in diesem Bereich aufmerksam zu machen, Gründe dafür ofenzulegen und das Arbeiten mit Cyanobakterien zugänglicher für Forschende außerhalb des eigenen Felds zu machen, um zur Entwicklung neuer cyanobakterieller Symbiosen mit neuen Spezies anzuregen. In einem weiteren Projekt wurden synthetische Konsortien zwischen Escherichia coli und Vibrio natriegens basierend auf Dependenz durch Aminosäure-Auxotrophien entwickelt. Es wurde eine starke Regulation der relativen Zelldichte beider Partner beobachtet, die resistent gegen externe und interne Störungen ist.While most of microbiological research focuses on the study of individual species, research on microbial consortia is still in its infancy. However, microbes in nature exist almost exclusively as part of such communities which can be found anywhere from the deep sea to hot springs, from the skin to the gut of humans. They are responsible for vital geo- and biochemical transformations and have been linked to Alzheimer9s and other diseases. Yet, very litle is known about the fundamental principles and dynamics of a microbial consortium. In order to truly understand individual species and their ecosystem it is essential to study them in the context of their interactions with their partners. This thesis aims to advance the oeld of microbial community research by advancing existing and creating novel model systems to investigate. In the first project we continued domestication of the natural colonizer of Hydra vulgaris, the bacterium Curvibacter sp. AEP1-3. Curvibacter is in an intimate cross talk with its host but also other members of the Hydra vulgaris microbiome. We established several new in trans expression systems and assessed their activity levels across diferent growth phases. In the second project we assessed the reproducibility of cyanobacterial experiments in an interlaboratory study across Europe. The aim was to raise awareness for reproducibility issues in cyanobacterial research, to make cyanobacteria more reproducible and to allow researchers outside of the phototroph oeld beter access to cyanobacteria as potential partners for symbiosis. In the third project we created synthetic inter- and intraspecies mutualistic relationships based on amino acid auxotrophy dependencies between Escherichia coli and Vibrio natriegens. We observe a strict maintenance of a cell-to-cell abundance ratio in these communities that is resistant to external and internal perturbations. | |||||||
| Quelle: | Beal, Jacob; Baldwin, Geof S.; Farny, Natalie G.; Gershater, Markus; Haddock-Angelli, Traci; Buckley-
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| Lizenz: |  Dieses Werk ist lizenziert unter einer Creative Commons Namensnennung 4.0 International Lizenz | |||||||
| Bezug: | Okt 2020 - Sep 2024 | |||||||
| Fachbereich / Einrichtung: | Mathematisch- Naturwissenschaftliche Fakultät » WE Biologie | |||||||
| Dokument erstellt am: | 07.10.2024 | |||||||
| Dateien geändert am: | 07.10.2024 | |||||||
| Promotionsantrag am: | 28.05.0024 | |||||||
| Datum der Promotion: | 02.09.2024 |
