Dokument: The Effect of Natural Genetic Variation at PPD-H1 on Shoot Apex Development in Response to High Ambient Temperature in Barley (Hordeum vulgare L.)
| Titel: | The Effect of Natural Genetic Variation at PPD-H1 on Shoot Apex Development in Response to High Ambient Temperature in Barley (Hordeum vulgare L.) | |||||||
| URL für Lesezeichen: | https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=66555 | |||||||
| URN (NBN): | urn:nbn:de:hbz:061-20250908-092531-1 | |||||||
| Kollektion: | Dissertationen | |||||||
| Sprache: | Englisch | |||||||
| Dokumententyp: | Wissenschaftliche Abschlussarbeiten » Dissertation | |||||||
| Medientyp: | Text | |||||||
| Autor: | Lan, Tianyu [Autor] | |||||||
| Dateien: |
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| Beitragende: | Prof. Dr. von Korff Schmising, Maria [Gutachter] Prof. Dr. Rüdiger Simon [Gutachter] | |||||||
| Stichwörter: | Hordeum vulgare, high ambient temperature, development | |||||||
| Dewey Dezimal-Klassifikation: | 500 Naturwissenschaften und Mathematik » 580 Pflanzen (Botanik) | |||||||
| Beschreibung: | Increases in global temperatures impair spike development and fertility, thus significantly reducing the productivity of temperate cereal crops, such as barley (Hordeum vulgare) and wheat (Triticum aestivum). A photoperiod response gene, PHOTOPERIOD1 (PPD-H1), has been identified as a major regulator of flowering time and seed set in response to ambient temperature variation. However, the mechanisms underlying the interaction between PPD-H1 and ambient temperatures in regulating plant growth, spike development and, thus, grain yield are poorly understood. The aim of my study was thus to dissect the effects of natural genetic variation at PPD-H1 on shoot growth and inflorescence development at different developmental stages and to identify genes and regulatory networks in the leaf and main shoot apices (MSA) associated with the temperature- and PPD-H1-dependent differences in developmental timing, leaf and floral development under HT in barley.
I dissected developmental and molecular differences between two pairs of introgression lines differing at PPD-H1 by scoring microscopic development of the shoot and conducting global transcriptome analyses in developing leaves and MSAs of parental and introgression lines grown under control (CT) and high ambient temperature (HT) conditions. I could demonstrate that Ppd-H1 interacted with HT to control the rate and duration of spikelet and floral meristem induction and, thus, inflorescence meristem activity and maintenance. Furthermore, HT impaired floret fertility by altering anther and pollen development in a genotype-specific manner, suggesting that Ppd-H1 could improve anther and pollen development under HT. My work also demonstrated that the vegetative MSA was more susceptible to HT treatment than the reproductive MSA as seen by the long-term effects of HT on the spikelet and floral meristem induction after transfer to CT. RNA-sequencing revealed that HT reduced the expression of floral inducers in the leaf and floral developmental regulators in the MSA. Differential expression of genes related to stress response, photosynthesis, and carbohydrate and energy metabolism suggested that the differences in the overall stress resistance, photosynthetic apparatus development, and energy metabolism underly the PPD-H1-dependent differences in floret fertility under HT. My study thus provides a thorough analysis of shoot developmental traits and the molecular mechanism that underlies the HT-induced grain yield reduction. Furthermore, the molecular analyses demonstrated that Ppd-H1 controls, in addition to developmental timing, overall stress resistance, thereby improving floret fertility and grain set under HT. | |||||||
| Lizenz: |  Dieses Werk ist lizenziert unter einer Creative Commons Namensnennung 4.0 International Lizenz | |||||||
| Fachbereich / Einrichtung: | Mathematisch- Naturwissenschaftliche Fakultät » WE Biologie » Genetik | |||||||
| Dokument erstellt am: | 08.09.2025 | |||||||
| Dateien geändert am: | 08.09.2025 | |||||||
| Promotionsantrag am: | 19.10.2023 | |||||||
| Datum der Promotion: | 12.12.2023 |
