Dokument: Receptor signaling specificity in plants
| Titel: | Receptor signaling specificity in plants | |||||||
| URL für Lesezeichen: | https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=55722 | |||||||
| URN (NBN): | urn:nbn:de:hbz:061-20210317-105255-7 | |||||||
| Kollektion: | Dissertationen | |||||||
| Sprache: | Englisch | |||||||
| Dokumententyp: | Wissenschaftliche Abschlussarbeiten » Dissertation | |||||||
| Medientyp: | Text | |||||||
| Autor: | Blümke, Patrick [Autor] | |||||||
| Dateien: |
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| Beitragende: | Prof. Dr. Simon, Rüdiger [Gutachter] Prof. Dr. Georg Groth [Gutachter] | |||||||
| Dewey Dezimal-Klassifikation: | 500 Naturwissenschaften und Mathematik » 570 Biowissenschaften; Biologie | |||||||
| Beschreibung: | CLAVATA family (CLVf) receptors are plasma membrane (PM)-located receptor-like kinases (RLKs) with key functions in various signaling pathways of Arabidopsis thaliana. CLVf receptors sense extracellular CLV3/EMBRYO SURROUNDING REGION-related (CLE) peptides by forming complexes with co-receptors and proteins like CLAVATA2 (CLV2) and CORYNE (CRN) at the PM. This study contributes to understand how CLE peptide signals are transmitted intracellularly after receptor activation at the PM. Furthermore, the study reveals mechanisms of pathway specificity explaining how the same set of CLVf receptors can mediate distinct signaling outputs in different cellular contexts.
Many RLK-based signaling pathways in plants are highly intertwined and cross-regulated. The review in the first chapter of this thesis presents recently reported principles that guarantee specific signaling within complex informational networks. Thereby, spatio-temporal receptor availability at the PM was identified as one of the main drivers for pathway specificity. A major advance of this study is the identification of the receptor-like cytoplasmic kinase MAZZA (MAZ) and its homologs as novel interactors of CLVf receptors. MAZ is reversibly PM-associated and shares expression domains with the CLVf genes. Genetic analyses revealed that MAZ functions during root meristem development and stomatal patterning. The latter displays a novel place of action of the CLVf receptor pathways. The study sheds light on the role of the CRN/CLV2 heteromer within root development. Previous reports demonstrated that CLE-triggered root meristem differentiation depends on CRN, which defines PM-localization of CLVf receptors. Tissue-specific CRN expression rescue assays in this study revealed that CRN can mediate the same developmental programs from distinct subdomains of the root meristem indicating non-cell-autonomous regulation. Furthermore, the study comprises new insights how precisely balanced subcellular protein compositions affect CLVf receptor signaling. OCTOPUS (OPS) displays polar localization in developing phloem cells in the root, where it competitively interferes the interaction between CRN/CLV2 and the CLVf receptor BARELY ANY MERISTEM3 (BAM3). Thereby, OPS dampens the pathway dosage-dependently and locally defined, displaying a novel regulatory mechanism for specificity in CLVf receptor signaling. To improve protein-protein interaction studies in future experiments, this thesis includes a guide for FRET pair selection with high performance at the PM of plant cells. Together, the thesis comprises several novel concepts on CLVf receptor pathways regulation, signal fine-tuning, and signal transmission. This contributes to the overall understanding of RLK-based informational networks in plants. | |||||||
| Lizenz: |  Urheberrechtsschutz | |||||||
| Fachbereich / Einrichtung: | Mathematisch- Naturwissenschaftliche Fakultät | |||||||
| Dokument erstellt am: | 17.03.2021 | |||||||
| Dateien geändert am: | 17.03.2021 | |||||||
| Promotionsantrag am: | 11.01.2021 | |||||||
| Datum der Promotion: | 18.02.2021 |
