Dokument: Development of optogenetic tools for plant synthetic biology
| Titel: | Development of optogenetic tools for plant synthetic biology | |||||||
| URL für Lesezeichen: | https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=54361 | |||||||
| URN (NBN): | urn:nbn:de:hbz:061-20211028-082907-5 | |||||||
| Kollektion: | Dissertationen | |||||||
| Sprache: | Englisch | |||||||
| Dokumententyp: | Wissenschaftliche Abschlussarbeiten » Dissertation | |||||||
| Medientyp: | Text | |||||||
| Autor: | Ochoa-Fernandez, Rocio [Autor] | |||||||
| Dateien: |
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| Beitragende: | Prof. Dr. Zurbriggen, Matias [Betreuer/Doktorvater] Prof. Dr. Simon, Rüdiger [Gutachter] | |||||||
| Dewey Dezimal-Klassifikation: | 500 Naturwissenschaften und Mathematik » 570 Biowissenschaften; Biologie | |||||||
| Beschreibung: | Plant synthetic biology is a nascent research area and, therefore, the development and implementation of engineering methods and synthetic tools still lags behind. In particular, optogenetic switches allow a precise quantitative regulation of cellular processes, such as gene expression, at high spatiotemporal resolution, overcoming limitations of classical chemically-inducible systems. While being widely applied in animal systems, their implementation in plants imposes a challenge.
In this thesis, some of the challenges of implementing optogenetic tools in plants are addressed. Firstly, Arabidopsis protoplasts are proposed as a platform to implement and characterize optogenetic tools. The development of tools to control gene expression in this cellular system, inducible by green, red and blue light, are then described, as well as some concepts for future switches. These optogenetic tools are designed as modular components that can be used to devise complex multi-chromatic tools to control gene expression. Finally, the development of a synthetic light-inducible system for the targeted control of gene expression in plants is presented. This system is based on red and blue light-controlled photoreceptors, which in combination turn gene expression OFF under white light and ON under red light. The characterization of this tool, termed Plant Usable Light Switch-Elements (PULSE), in plant cells is shown as well as its implementation in leaf tissue, enabling the first steps towards its application in stable transformed plant lines. This work reflects on the development of these first optogenetic systems for plants and stress on the novel perspectives they present for the study of plant signalling processes, such as the analysis of complex regulatory systems and metabolic pathways, with minimized invasiveness and high spatiotemporal resolution. | |||||||
| Lizenz: |  Urheberrechtsschutz | |||||||
| Fachbereich / Einrichtung: | Mathematisch- Naturwissenschaftliche Fakultät | |||||||
| Dokument erstellt am: | 28.10.2021 | |||||||
| Dateien geändert am: | 28.10.2021 | |||||||
| Promotionsantrag am: | 19.12.2019 | |||||||
| Datum der Promotion: | 07.07.2020 |
