Dokument: Elucidate the important role of peroxisomal transport proteins for plant growth and development
| Titel: | Elucidate the important role of peroxisomal transport proteins for plant growth and development | |||||||
| URL für Lesezeichen: | https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=53537 | |||||||
| URN (NBN): | urn:nbn:de:hbz:061-20210715-082707-7 | |||||||
| Kollektion: | Dissertationen | |||||||
| Sprache: | Englisch | |||||||
| Dokumententyp: | Wissenschaftliche Abschlussarbeiten » Dissertation | |||||||
| Medientyp: | Text | |||||||
| Autor: | Charton, Lennart [Autor] | |||||||
| Dateien: |
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| Beitragende: | Apl. Prof. Dr. Linka, Nicole [Gutachter] Prof. Dr. Groth, Georg [Gutachter] | |||||||
| Dewey Dezimal-Klassifikation: | 500 Naturwissenschaften und Mathematik » 580 Pflanzen (Botanik) | |||||||
| Beschreibung: | Plant cells are compartmentalized into different organelles separated by membranes, which enables the simultaneous implementation of specialized metabolic reactions. But substrates have to be transported across these barriers in an efficient and controllable way, since many metabolic pathways are shared between different organelles.
Peroxisomes play a central role in a plethora of metabolic reactions covering all stages of plant development from seedling establishment over photosynthesis to reproduction and senescence. But the majority of the transport proteins required for these processes are still unknown. The mitochondrial carrier family (MCF) represents one of the largest and most diverse group of transport proteins in humans, yeast and plants. Despite their name representatives of this protein family have been shown to be localized to other organelles like chloroplasts, the endoplasmic reticulum and peroxisomes. In total, only five peroxisomal membrane transport proteins have been identified and characterized in plants with four of them belonging to the MCF indicating that this family have played a special role in the development of peroxisomal transport proteins. This thesis deals with the characterization of four novel peroxisomal MCF carrier that have not been analyzed in detail before. Three of them share high sequence similarity to each other and to already characterized Coenzyme A carrier and thus are likely to have redundant function in supplying peroxisomes with CoA. Hence, we call them peroxisomal CoA carrier (PCC) 1, 2 and 3. They are up-regulated in CoA dependent processes like germination and upon wounding. And at least PCC1 can complement yeast mutants defective in peroxisomal NAD redox shuttles, which is interesting, since NAD share high structural similarity with CoA. The fourth unknown novel peroxisomal member of the MCF is a heat shock responsive carrier (PHS1). Plants deficient in PHS1 are more susceptible to conditions of elevated oxidative stress and more precisely to elevated temperatures as a major cause of plant oxidative stress. In addition, we aimed to further analyze the importance of the already identified peroxisomal ATP carrier PNC1 and PNC2 beyond their known role in -oxidation. We could show the importance of ATP supply mediated by the PNC proteins for the synthesis of plant hormones via the mevalonate pathway. Moreover, analysis of the in vivo ATP pool in the pnc1/2 double mutant using state of the art biosensors revealed an overall lower energy level in the mutant compared to the WT. Other putative functions of the PNC proteins are discussed including their potential role in photorespiration by supplying ATP for regulatory processes mediated by kinases as well as response to biotic and abiotic stresses. | |||||||
| Lizenz: |  Urheberrechtsschutz | |||||||
| Fachbereich / Einrichtung: | Mathematisch- Naturwissenschaftliche Fakultät » WE Biologie » Biochemie der Pflanzen | |||||||
| Dokument erstellt am: | 15.07.2021 | |||||||
| Dateien geändert am: | 15.07.2021 | |||||||
| Promotionsantrag am: | 14.01.2020 | |||||||
| Datum der Promotion: | 16.03.2020 |
