Dokument: Studies into the genetic architecture of C3-C4 characteristic in Moricandia

Titel:	Studies into the genetic architecture of C3-C4 characteristic in Moricandia
URL für Lesezeichen:	https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=53366
URN (NBN):	urn:nbn:de:hbz:061-20200608-150130-1
Kollektion:	Dissertationen
Sprache:	Englisch
Dokumententyp:	Wissenschaftliche Abschlussarbeiten » Dissertation
Medientyp:	Text
Autor:	Lin, Meng-Ying [Autor]
Dateien:	[Dateien anzeigen] Adobe PDF [Details] 11,45 MB in einer Datei [ZIP-Datei erzeugen] Dateien vom 05.06.2020 / geändert 05.06.2020
Beitragende:	Prof. Dr. Weber, Andreas [Gutachter] Prof. Dr. Stich, Benjamin [Gutachter]
Dewey Dezimal-Klassifikation:	500 Naturwissenschaften und Mathematik » 570 Biowissenschaften; Biologie
Beschreibung:	C3-C4 intermediate species are of great value to unravel molecular mechanisms of the early evolutionary steps of C4 photosynthesis, owing to the fact that they are predicted to be natural occurring intermediates on the convergent evolution from C3 to C4 photosynthesis. C3-C4 plants possess Kranz-like anatomy, bundle sheath cells with numerous organelles arranged centripetally around the veins, which serves as a requisite for the photorespiratory glycine shuttle. The P-subunit of the photorespiratory enzyme glycine decarboxylase is absent in C3-C4 mesophyll cells. In order to digest the toxic product 2-phosphogylcolate, the photorespiratory glycine is shuttled from mesophyll to bundle sheath cells, where the released CO2 can be efficiently recaptured by numerous, adjacent chloroplasts. Transcriptional regulation of spatial expression pattern of the P-protein of glycine decarboxylase between C3 and C3-C4 species is well known in Flaveria and Moricandia. However, the genetic architecture of C3-C4 characteristics remains largely elusive. In this thesis, I investigated cytogenetic characteristics of Moricandia C3 and C3-C4 species and the potential of interspecific hybridization between C3 and C3-C4 plant, serving as fundamental knowledge for further transcriptional regulation analysis and comparative transcriptomics. Different interspecific hybridizations between C3 and C3-C4 species in Moricandia were performed, where the hybridized pair of C3-C4 M. arvensis as maternal and C3 M. moricandioides as paternal species was the most prolific combination. To investigate C3-C4 intermediate specific gene expression, allele specific expression analysis was applied to the interspecific hybrids of M. arvensis and M. moricandioides. It demonstrated that cis-mechanisms play an important role in shaping C3 to C3-C4 photosynthesis type and the corresponding transcripts are enriched in major photosynthetic pathways and chloroplast relocation. Candidates with strong allele specific expression are of special interest for studying the genetic architecture regarding C3-C4 characteristic traits. Comparison of anatomical changes during leaf ontology between C3 and C3-C4 Moricandia species revealed the early establishment of Kranz-like anatomy in C3-C4 species. Through the comparative transcriptome and cluster analysis during leaf development of closely related C3 and C3-C4 Moricandia species, we found transcription factors as potential candidates for genetic control of C3-C4 characteristics, such as auxin response, sigma-like, and growth-related factor, and a cohort of genes regulating vein initiation, procambium formation, xylem formation, SCR/SHR pathway, and vein patterning associated with the early leaf development in C3-C4 M. arvensis. The early organelle accumulation in C3-C4 bundle sheath cells was more relevant to plastid division than formation and biosynthesis.
Lizenz:	Urheberrechtsschutz
Fachbereich / Einrichtung:	Mathematisch- Naturwissenschaftliche Fakultät » WE Biologie » Biochemie der Pflanzen
Dokument erstellt am:	08.06.2020
Dateien geändert am:	08.06.2020
Promotionsantrag am:	09.01.2020
Datum der Promotion:	05.05.2020