Dokument: Punctual acoustic-wave therapy for the super-selective ablation of squamous cell carcinoma
| Titel: | Punctual acoustic-wave therapy for the super-selective ablation of squamous cell carcinoma | |||||||
| URL für Lesezeichen: | https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=48379 | |||||||
| URN (NBN): | urn:nbn:de:hbz:061-20210305-091227-5 | |||||||
| Kollektion: | Dissertationen | |||||||
| Sprache: | Englisch | |||||||
| Dokumententyp: | Wissenschaftliche Abschlussarbeiten » Dissertation | |||||||
| Medientyp: | Text | |||||||
| Autor: | Strugačevac, Maja [Autor] | |||||||
| Dateien: |
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| Beitragende: | Prof. Dr. Getzlaff, Mathias [Gutachter] Prof. Dr. Dr. Schipper, Jörg [Gutachter] | |||||||
| Dewey Dezimal-Klassifikation: | 500 Naturwissenschaften und Mathematik » 570 Biowissenschaften; Biologie | |||||||
| Beschreibung: | This dissertation summarizes the author’s work on the development of new, cell-selective treatment strategies for tumors originating from the head and neck area. This work comprises of three main parts: determination of suitable staining parameters for the observed cell lines, characterization of the cells and acoustic wave irradiation.
As all the measurements were made using a fluorescence confocal laser scanning microscope, the suitable staining concentration and staining time had to be determined. In this way, the image quality can be ensured and the influence of the method used on the cells can be minimized. In a second part the morphology of the cells was observed to find the differences between healthy, dysplastic and cancerous cells. The differences in cytoskeleton filaments may lead to the differences in the elasticity of the cells which is of great importance for the development of a new cancer treatment based on the mechanical properties of the cells. The third part of this thesis deals with the irradiation of the cells using acoustic waves exhibiting frequencies from 0.5 kHz to 10.0 kHz. The main aim was to observe whether those acoustic waves can induce a resonance catastrophe which would lead to a cell death. The reaction of the cells on the variation of the frequency, the oscillation amplitude, the irradiation duration and the probe-cells distance was observed to investigate whether the change of those parameters induces different reactions of the cells. The parameter set on which the cells react the most was determined. The observed cancer and dysplastic cells were then irradiated using this parameters to examine the reaction of different cell lines on the same parameter set. The different reaction of the cells indicates that the therapy has to be adjusted for every patient individually. This thesis presents fundamental results of great importance for further research and the development of super-selective ablation of cancer cells. | |||||||
| Lizenz: |  Urheberrechtsschutz | |||||||
| Fachbereich / Einrichtung: | Mathematisch- Naturwissenschaftliche Fakultät » WE Physik » Angewandte Physik | |||||||
| Dokument erstellt am: | 05.03.2021 | |||||||
| Dateien geändert am: | 05.03.2021 | |||||||
| Promotionsantrag am: | 19.11.2018 | |||||||
| Datum der Promotion: | 23.01.2019 |
