Dokument: Connecting metabolic networks - The role of the Mitochondrial Carrier Family in plants
| Titel: | Connecting metabolic networks - The role of the Mitochondrial Carrier Family in plants | |||||||
| URL für Lesezeichen: | https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=48166 | |||||||
| URN (NBN): | urn:nbn:de:hbz:061-20190103-091215-4 | |||||||
| Kollektion: | Dissertationen | |||||||
| Sprache: | Englisch | |||||||
| Dokumententyp: | Wissenschaftliche Abschlussarbeiten » Dissertation | |||||||
| Medientyp: | Text | |||||||
| Autor: | M.Sc. Hielscher, Björn [Autor] | |||||||
| Dateien: |
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| Beitragende: | Dr. Linka, Nicole [Gutachter] Prof. Dr. Westhoff, Peter [Gutachter] | |||||||
| Stichwörter: | Mitochondrial Carrier Family, MCF, Photorespiration, β-Oxidation, Dicarbonsäuren, Peroxisomen, Mitochondrien | |||||||
| Dewey Dezimal-Klassifikation: | 500 Naturwissenschaften und Mathematik » 580 Pflanzen (Botanik) | |||||||
| Beschreibung: | This thesis is divided into independent sections with two submission-ready manuscripts and three previously published works. Manuscript 1 describes the identification and biochemical characterization of two transport proteins probably involved in photorespiration and β-oxidation. Comparative co-expression analysis of C3, C4 and intermediate Flaveria transcriptomes was used to identify 34 candidate genes linked to photorespiration in Arabidopsis. Two selected Arabidopsis proteins from the mitochondrial carrier family (MCF) localized in peroxisomes were biochemically characterized. Arabidopsis loss-of-function mutants were established using the CRISPR/Cas9 system. Manuscript 2 focuses on the phenotypic analysis of two Arabidopsis T-DNA insertion lines impaired in the expression of the MCF member and mitochondrial phosphate transporter AtPHT3;3. Each manuscript concludes with an outlook section. Both manuscripts are included in section V. Section VI summarizes the results of Manuscript 1 and 2. Section VII contains three previously published manuscripts. Manuscript 3 is a method paper on the analysis of peroxisomal β-oxidation in Arabidopsis seeds during early germination published as Hielscher et al. (2017). Manuscript 4 describes the biochemical characterization of the Arabidopsis MCF transporters AtUCP1 and AtUCP2 and was published as Monné et al. (2018). Manuscript 5 describes the function of two Cichorium intybus enzymes CiSHT1 and CiSHT2 and was published as Delporte et al. (2018). | |||||||
| Quelle: | Delporte, M., Bernard, G., Legrand, G., Hielscher, B., Lanoue, A., Molinié, R., Rambaud, C., Mathiron, D., Besseau, S., Linka, N., Hilbert, J.-L., and Gagneul, D. (2018). A BAHD neofunctionalization promotes tetrahydroxycinnamoyl spermine accumulation in the pollen coats of the Asteraceae family. J. Exp. Bot. 69: 5355–5371.
Hielscher, B., Charton, L., Mettler-Altmann, T., and Linka, N. (2017). Analysis of Peroxisomal β-Oxidation During Storage Oil Mobilization in Arabidopsis thaliana Seedlings. Methods Mol. Biol. 1595: 291–304. Monné, M., Daddabbo, L., Gagneul, D., Obata, T., Hielscher, B., Palmieri, L., Miniero, D.V., Fernie, A.R., Weber, A.P.M., and Palmieri, F. (2018). Uncoupling proteins 1 and 2 (UCP1 and UCP2) from Arabidopsis thaliana are mitochondrial transporters of aspartate, glutamate, and dicarboxylates. J. Biol. Chem. 293: 4213–4227. | |||||||
| Lizenz: |  Urheberrechtsschutz | |||||||
| Fachbereich / Einrichtung: | Mathematisch- Naturwissenschaftliche Fakultät » WE Biologie » Biochemie der Pflanzen | |||||||
| Dokument erstellt am: | 03.01.2019 | |||||||
| Dateien geändert am: | 03.01.2019 | |||||||
| Promotionsantrag am: | 17.08.2018 | |||||||
| Datum der Promotion: | 17.12.2018 |
