Dokument: Structure-functional analysis of Stardust in the Drosophila eye
| Titel: | Structure-functional analysis of Stardust in the Drosophila eye | |||||||
| URL für Lesezeichen: | https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=4792 | |||||||
| URN (NBN): | urn:nbn:de:hbz:061-20080108-141049-9 | |||||||
| Kollektion: | Dissertationen | |||||||
| Sprache: | Englisch | |||||||
| Dokumententyp: | Wissenschaftliche Abschlussarbeiten » Dissertation | |||||||
| Medientyp: | Text | |||||||
| Autor: | Bulgakova, Natalia [Autor] | |||||||
| Dateien: |
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| Beitragende: | Prof. Dr. Knust, Elisabeth [Gutachter] Prof. Dr. Hegemann, J. H. [Gutachter] | |||||||
| Dewey Dezimal-Klassifikation: | 500 Naturwissenschaften und Mathematik » 570 Biowissenschaften; Biologie | |||||||
| Beschreibung: | The Crumbs- (Crb-) complex is required for two independent processes in the Drosophila eye. It assures the proper morphogenesis of the apical membrane of PRCs, and it prevents light-induced retinal degeneration in adult flies. Stardust (Sdt) plays a key role in the regulation of the different functions of the Crb-complex in the Drosophila eye. Three major functions of Sdt have been demonstrated in this work:
1. Sdt provides a link between the transmembrane Crb protein and other members of the complex as DPATJ, DLin-7 and, probably, DPar-6. It recruits them to the stalk membrane in adult eyes. In pupal stage, it is not required for localization of DPATJ, but may still be important for its proper connection to Crb. The connection between different members of the Crb-complex is required for proper morphogenesis of photoreceptor cells and for prevention of light-induced degeneration. 2. Sdt is crucial for the stabilization of the complex at the stalk membrane in the adult flies. For this function, its L27-N, PDZ, SH3 and GUK domains are necessary. At pupal stages, Sdt is not required for the stabilization of the Crb-complex at the apical membrane. The change in the requirement for Sdt protein is likely to occur when the apical membrane becomes subdivided into rhabdomere and stalk. 3. Sdt is expressed in several splice-variants, both at pupal and adult stages. The alternative splice-variants might provide the fine-tuning of the Crb-complex composition, and thus provide its proper function at different stages in the retina. Two additional interesting aspects came out of this work. First, the requirement of Sdt for proper localization of the Crb-complex is different before the stalk membrane formation and after. Before 60% p.d., Sdt has only minor function. Both Crb and DPATJ are localized properly. In adult photoreceptor cells, Sdt is crucial for localization of the cytoplasmic members and for the stability of the Crb-complex. Second, the Crb-complex might have a function not only in photoreceptor cells, but also in pigment cells, and the mechanisms controlled by the Crb-complex in different cell types may be different. In pigment cells, a link between Crb and DLin-7 provided by Sdt is necessary for prevention of light-induced retinal degeneration. In photoreceptor cells, a link between Crb and DPATJ provided by Sdt is required for this function. | |||||||
| Rechtliche Vermerke: | Aus patentrechtlichen Gründen bis 01.01.2008 zurückgestellt | |||||||
| Lizenz: |  Urheberrechtsschutz | |||||||
| Fachbereich / Einrichtung: | Mathematisch- Naturwissenschaftliche Fakultät » WE Biologie » Genetik | |||||||
| Dokument erstellt am: | 06.06.2007 | |||||||
| Dateien geändert am: | 06.06.2007 | |||||||
| Promotionsantrag am: | 02.04.2007 | |||||||
| Datum der Promotion: | 20.04.2007 |
