Dokument: Blue Light Photoreceptors - From Mechanism to Application
| Titel: | Blue Light Photoreceptors - From Mechanism to Application | |||||||
| URL für Lesezeichen: | https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=26944 | |||||||
| URN (NBN): | urn:nbn:de:hbz:061-20130920-100402-2 | |||||||
| Kollektion: | Dissertationen | |||||||
| Sprache: | Englisch | |||||||
| Dokumententyp: | Wissenschaftliche Abschlussarbeiten » Dissertation | |||||||
| Medientyp: | Text | |||||||
| Autor: | Raffelberg, Sarah [Autor] | |||||||
| Dateien: |
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| Beitragende: | Prof. Dr. Gärtner, Wolfgang [Gutachter] Prof. Dr. Jaeger, Karl-Erich [Gutachter] | |||||||
| Stichwörter: | Proteinbiochemie, Photorezeptoren, Mutagenese, Spektroskopie | |||||||
| Dewey Dezimal-Klassifikation: | 500 Naturwissenschaften und Mathematik » 540 Chemie | |||||||
| Beschreibung: | Light sensing photoreceptors connect the environment and a living organism by transforming outer stimuli (light) into a physiological signal. They are important to sense and to react to light, sensing spectral quality, intensity, direction and its periodicity. Scientific information on photoreceptors is continuously growing since several decades. Many big steps could be made and many small details were revealed, both pushing forward the knowledge about light sensitive systems and to offer the basic principle for endless possibilities in the field of applications.
This thesis is focused to open the path from fundamental knowledge on blue light sensitive photoreceptor function to the development of applications. Within the scope of this work the following proteins were investigated and the following questions were addressed: • What effects have the residues of the chromophore binding pocket on the photochemistry of the blue light photoreceptor YtvA from Bacillus subtilis? Which role plays the hydrogen bonding network for the regulation of the photocycle? Do other nearby residues influence the spectroscopic properties of the protein? Different variants of YtvA were generated via site-directed mutagenesis to analyze their spectroscopic behavior. • How does the protein structure of YtvA look like? Spin labeled YtvA mutants were used for EPR measurements to calculate a full-length protein structure. • Is the blue light receptor YtvA a good fluorescent reporter? Is it able to be converted into dark state by photoswitchable phenomenon? These experiments reveal the high potential of blue light receptors for physical applications like super-resolution microscopy (nanoscopy). • Can genome-mining be useful to detect and generate novel photoreceptors? A novel blue light-driven AMP cyclase from Microcoleus chthonoplastes was found and characterized here for the first time. In contrast to formerly reported AMP cyclases that are regulated through a blue light-sensing BLUF domain this novel proteins carries a LOV domain. | |||||||
| Lizenz: |  Urheberrechtsschutz | |||||||
| Fachbereich / Einrichtung: | Mathematisch- Naturwissenschaftliche Fakultät | |||||||
| Dokument erstellt am: | 20.09.2013 | |||||||
| Dateien geändert am: | 20.09.2013 | |||||||
| Promotionsantrag am: | 17.05.2013 | |||||||
| Datum der Promotion: | 21.06.2013 |
